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| Status |
Public on Jan 27, 2023 |
| Title |
Retinoic acid signaling modulation guides in vitro specification of human heart field-specific progenitor pools |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
He we report a systematic analysis of RA impact on human cardiovascular progenitor specification in orchestry with other signaling cues during early mesoderm formation. To approach this, we used a single cell transciptome profiling to dissect heterogeneity within human heart field-like cell populations and their derivatives. Our analysis enable us to identify known as well as a novel population of cells that can in vitro differentaite towards myocytic as well as epicardial lineages.
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| |
|
| Overall design |
single cell transcriptome analysis of hESC in vitro derived human primitive streak-like cells (d1.5), cardiovascular progenitors (4 sorted samples at d4.5) and their derivatives (4 samples at d30)
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| |
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| Contributor(s) |
Santamaria G, Zawada D |
| Citation(s) |
37012244 |
| |
| Submission date |
Mar 01, 2022 |
| Last update date |
Apr 18, 2023 |
| Contact name |
Gianluca Santamaria |
| E-mail(s) |
santamariagianluca@tum.de
|
| Organization name |
Klinikum rechts der Isar – Technical University Munich
|
| Department |
Department of Medicine I
|
| Lab |
Molecular Cardiology
|
| Street address |
Ismaninger Strasse 22
|
| City |
Munich |
| State/province |
Germany |
| ZIP/Postal code |
81675 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (9)
|
| GSM5924609 |
Cardiovascular progenitor cells (FACS d4.5 RA- eGFP+) |
| GSM5924610 |
Cardiovascular progenitor cells (FACS d4.5 RA+ eGFP+) |
| GSM5924611 |
Cardiovascular progenitor cells (FACS d4.5 RA+ mCherry+ eGFP+) |
| GSM5924612 |
Cardiovascular progenitor cells (FACS d4.5 RA+ mCherry+) |
| GSM5924613 |
Primitive streak-like cells (d1.5) |
| GSM5924614 |
Cardiovascular progenitor cells (FACS d4.5 RA- eGFP+) (d30) |
| GSM5924615 |
Aggregates of FACS d4.5 RA+ eGFP+ (d30) |
| GSM5924616 |
Aggregates of FACS d4.5 RA+ mCherry+ eGFP+ (d30) |
| GSM5924617 |
Aggregates of FACS d4.5 RA+ mCherry (d30) |
|
| Relations |
| BioProject |
PRJNA811512 |
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