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Status |
Public on Mar 09, 2023 |
Title |
Effect of SigA depletion and SigB knockout on the global transcription of Mtb |
Organism |
Mycobacterium tuberculosis |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Analyzing differences in transcriptome profile of RvΔsigA (SigA conditional knockdown strain) in presence of anhydrotetracycline and transcriptome profile of RvΔsigB (SigB knockout strain) compared to control.
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Overall design |
RvΔsigA was inoculated at 0.05 OD, in presence or absence of 1mM/ml anhydrotetracycline. Post 3 days total RNA was islolated and RNA seq was performed. RvΔsigB was inoculated at 0.05 OD and grown to 0.8 OD, after which RNA was isolated and RNA sequencing was carried out.
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Web link |
https://doi.org/10.1016/j.jbc.2023.102933
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Contributor(s) |
Singha B, singh Nk, Sowpati DT, Nandicoori VK |
Citation(s) |
36690275 |
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Submission date |
Mar 02, 2022 |
Last update date |
Mar 10, 2023 |
Contact name |
Nitesh Kumar Singh |
E-mail(s) |
nitesh@ccmb.res.in
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Organization name |
Center for Cellular and Molecular Biology
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Department |
Bioinformatics
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Street address |
Habsiguda
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City |
Hyderabad |
State/province |
Telangana |
ZIP/Postal code |
500007 |
Country |
India |
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Platforms (1) |
GPL27507 |
Illumina NovaSeq 6000 (Mycobacterium tuberculosis) |
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Samples (10)
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Relations |
BioProject |
PRJNA811806 |