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Series GSE197746 Query DataSets for GSE197746
Status Public on Nov 19, 2022
Title Hepatic DKK1 driven steatosis is CD36-dependent
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary With the improvement of people's living standards and lifestyle changes, nonalcoholic fatty liver disease (NAFLD) has become one of the most common chronic liver diseases worldwide. However, few drugs are available for NAFLD, partly due to an incomplete understanding of its pathogenic mechanisms. Here, using in vivo and in vitro gain- and loss-of function approaches, we identified DKK1 as a pivotal mediator of the progression of NAFLD and its accompanying metabolic disorders in dietary obese mice. Mechanistic study reveals that DKK1 enhances the capacity of hepatocytes to uptake fatty acids through ERK-PPARĪ³-CD36 pathway. Moreover, DKK1 increased insulin resistance by activating the JNK signaling pathway, which in turn exacerbates disorders of hepatic lipid metabolism. These results suggest that DKK1 is a regulator of fatty acid uptake in lipid metabolism and insulin signaling, and may be a potential therapeutic candidate for NAFLD
 
Overall design hepatocyte mRNA profiles of overexpression DKK1 (OE-DKK1) and green fluorescent protein negative control (GFP-NC) AML12 cells treated with 0.5mM free fatty acid (FFA ) for 24 hours.
 
Contributor(s) Yang Z, Li Y
Citation(s) 36410795
Submission date Mar 02, 2022
Last update date Dec 09, 2022
Contact name Yang Zhen
Organization name Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
Lab li_yinxiong
Street address 190 Kaiyuan Avenue, Science Park,
City Guangzhou
State/province China/Guangdong
ZIP/Postal code 510530
Country China
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM5929374 AML12 cells, AGF5
GSM5929375 AML12 cells, AOF5
Relations
BioProject PRJNA811955

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Supplementary file Size Download File type/resource
GSE197746_all.fpkm_anno.txt.gz 1.9 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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