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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 01, 2011 |
| Title |
The soluble intracellular domain of megalin does not affect renal proximal tubular function in vivo |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The endocytic receptor megalin constitutes the main pathway for clearance of plasma proteins from the glomerular filtrate in the proximal tubules. However, little is know about the mechanisms that control receptor activity. A widely discussed hypothesis states that the intracellular domain (ICD) of megalin, released upon ligand binding, acts as a transcription regulator to suppress receptor expression - a mechanism proposed to safeguard the proximal tubules from protein overload. Here, we have put this hypothesis to the test by generating a mouse model co-expressing the soluble ICD and the full-length receptor. Despite pronounced expression in the proximal tubules, the ICD failed to exert any effects on renal proximal tubular function such as megalin expression, protein retrieval, or renal gene transcription. Thus, our data argue that the ICD does not play a role in regulation of megalin activity in vivo in the proximal tubules.
We used microarrays to compare gene expression profile in adult kidney from a new mouse model expressing the intracellular domain of megalin with wildtype.
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| Overall design |
10 week old mice were collected for RNA extraction and hybridization on Affymetrix microarrays. Three individuals for each genotype were analyzed comparing heterozygous animals for the intracellular domain of megalin with littermates controls.
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| Contributor(s) |
Christ A, Terryn S, Christensen EI, Huska MR, Andrade-Navarro MA, Hübner N, Devuyst O, Hammes A, Willnow TE |
| Citation(s) |
20531455 |
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| Submission date |
Jan 07, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Thomas E Willnow |
| E-mail(s) |
willnow@mdc-berlin.de
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| Organization name |
Max Delbrück Center for Molecular Medicine (MDC)
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| Lab |
AG Willnow
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| Street address |
Robert-Rössle-Str. 10
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| City |
Berlin |
| State/province |
Berlin |
| ZIP/Postal code |
D-13125 |
| Country |
Germany |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| GSM493930 |
adult 10 week old, biological rep 1, TG |
| GSM493931 |
adult 10 week old, biological rep 2, TG |
| GSM493932 |
adult 10 week old, biological rep 3, TG |
| GSM493933 |
adult 10 week old, biological rep 1, WT |
| GSM493934 |
adult 10 week old, biological rep 2, WT |
| GSM493935 |
adult 10 week old, biological rep 3, WT |
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| Relations |
| BioProject |
PRJNA121863 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19778_RAW.tar |
22.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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