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Status |
Public on Mar 04, 2010 |
Title |
Neuronal MeCP2 is expressed at near histone-octamer levels and globally alters the chromatin state |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing Methylation profiling by high throughput sequencing
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Summary |
We report the application of single molecule-based sequencing technology for high-throughput genom-wide mapping of MeCP2 binding and DNA methylation in mouse brain and cerebellum respectively. We find a good correlation between MeCP2 occupancy and methyl-CpG density and depletion of MeCP2 binding at CpG Islands, the majority of which are constitutively hypomethylated. This study provides a comprehensive characterisation of the genome wide distribution of MeCP2.
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Overall design |
Examination of MeCP2 occupancy in mouse brain and the distribution of methylation in mouse cerebellum.
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Contributor(s) |
Skene PJ, Illingworth RS, Webb S, Kerr A, James KD, Turner DJ, Andrews R, Bird AP |
Citation(s) |
20188665 |
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Submission date |
Jan 07, 2010 |
Last update date |
May 15, 2019 |
Contact name |
Adrian P Bird |
E-mail(s) |
A.Bird@ed.ac.uk
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Phone |
0131 6508695
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Fax |
0131 6505379
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Organization name |
The University of Edinburgh
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Department |
WTCCB
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Lab |
Bird Lab
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Street address |
Mayfield Road
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City |
Edinburgh |
State/province |
Midlothian |
ZIP/Postal code |
EH9 3JR |
Country |
United Kingdom |
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Platforms (1) |
GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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Samples (2) |
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Relations |
SRA |
SRP002061 |
BioProject |
PRJNA122117 |