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Status |
Public on Jan 01, 2023 |
Title |
Transcriptomic study identifies quercetin and rutin as repurposed drugs for Parkinson’s Disease in in vitro 6-OHDA model |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
SH-SY5Y cells were treated with 6-hydroxydopamine (6-OHDA) to obtain an in vitro PD model. Transcriptome data were collected from both treated and untreated cells.We mapped significantly upregulated genes on a protein-protein interaction network to identify potential drug targets. Drugs targeting the selected drug targets were applied to 6-OHDA treated SH-SY5Y cells. Among the candidate drugs, quercetin and rutin were shown to be neuroprotective by effectively decreasing cell death.
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Overall design |
In total, 16 independent experiments were performed to obtain 8 untreated samples and 8 6-OHDA treated samples. Samples were collected at 12th and 18th hours from both treated and control experiments. Each group was run in four replicates.
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Contributor(s) |
Yigit EN, Sonmez E, Yuksel I, Cakir T, Aksan Kurnaz I |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
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Submission date |
Mar 06, 2022 |
Last update date |
Jan 03, 2023 |
Contact name |
Tunahan Cakir |
E-mail(s) |
tcakir@gtu.edu.tr
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Organization name |
Gebze Technical University
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Department |
Department of Bioengineering
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Street address |
Gebze, Kocaeli, Turkey
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City |
Gebze |
State/province |
Kocaeli |
ZIP/Postal code |
41400 |
Country |
Turkey |
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Platforms (1) |
GPL20844 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Feature Number Version] |
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Samples (16)
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GSM5935751 |
SH-SY5Y, Control, 12hr, rep4 |
GSM5935752 |
SH-SY5Y, Control, 18hr, rep1 |
GSM5935753 |
SH-SY5Y, Control, 18hr, rep2 |
GSM5935754 |
SH-SY5Y, Control, 18hr, rep3 |
GSM5935755 |
SH-SY5Y, Control, 18hr, rep4 |
GSM5935756 |
SH-SY5Y, 6OHDA, 12hr, rep1 |
GSM5935757 |
SH-SY5Y, 6OHDA, 12hr, rep2 |
GSM5935758 |
SH-SY5Y, 6OHDA, 12hr, rep3 |
GSM5935759 |
SH-SY5Y, 6OHDA, 12hr, rep4 |
GSM5935760 |
SH-SY5Y, 6OHDA, 18hr, rep1 |
GSM5935761 |
SH-SY5Y, 6OHDA, 18hr, rep2 |
GSM5935762 |
SH-SY5Y, 6OHDA, 18hr, rep3 |
GSM5935763 |
SH-SY5Y, 6OHDA, 18hr, rep4 |
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Relations |
BioProject |
PRJNA813345 |
Supplementary file |
Size |
Download |
File type/resource |
GSE198009_RAW.tar |
59.3 Mb |
(http)(custom) |
TAR (of TXT) |
Processed data included within Sample table |
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