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| Status |
Public on Mar 14, 2022 |
| Title |
Study on the Expression of miRNA in Exosome of EBV-positive Gastric Carcinoma Cells |
| Organisms |
Homo sapiens; human gammaherpesvirus 4 |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
We investigate the expression of miRNA in exosome of EBV-positive gastric carcinoma cells. The exosomes of EBV-positive and negative gastric carcinoma cells were separated by ultracentrifugation, the morphology of exosomes was identified by transmission electron microscopy, the exosome size was analyzed by Nanosight, and the expression of exosome membrane protein CD63 and CD81 was detected by western blot. High-throughput sequencing was used to detect miRNA expression profiles in gastric cancer cell lines and their exosomes. Under the ultra-microscopic electron microscope, the exosomes are seen as a typical translucent cup-like structure or a flat spherical structure. The nanoparticle tracking analyzer (Nanosight) showed that the exosomes were between 30 and 150 nm in diameter. Western blot(WB) assays showed that exosomes secreted by EBVaGC and EBVnGC cells expressed specific exosome membrane-associated proteins CD63 and CD81. High-throughput sequencing revealed that EBVaGC(SNU-719) and EBVnGC(AGS) and their secreted exosomes were highly expressed with certain human miRNAs, among which AGS-exo was highly expressed with hsa-miR-23b-3p, hsa-miR-320a-3p, and hsa-miR-4521. SNU-719-exo was highly expressed as hsa-miR-21-5p, hsa-miR-148a-3p and hsa-miR-7-5p. Nearly all EBV-related miRNAs (EBV-miRNA) were expressed in SNU-719 cells and their exosomes, among which EBV-miR-BART1-5p, EBV-miR-BART17-3p and EBV-miR-BART18-5p were the highest in SNU-719 cells, EBV-miR-BART1-5p, EBV-miR-BART18-5p and EBV-miR-BART17-3p were the highest in SNU-719-exo.
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| Overall design |
Examination of the expression of miRNA in exosome of EBV-positive gastric carcinoma cells
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| Contributor(s) |
Lin M, Zhang T, Li J, Gao F, Zheng K, Hu S, Li G, Ren C, Chen X, Guo F, Zhang S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Mar 10, 2022 |
| Last update date |
Mar 15, 2022 |
| Contact name |
Sheng Zhang |
| E-mail(s) |
zhgshg@fjmu.edu.cn
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| Organization name |
The First Affiliated Hospital of Fujian Medical University
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| Department |
Department of Pathology
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| Street address |
20 Cha Zhong Lu
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| City |
Fuzhou |
| State/province |
Fujian |
| ZIP/Postal code |
350004 |
| Country |
China |
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| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL23185 |
Illumina HiSeq 2500 (Homo sapiens; Human gammaherpesvirus 4) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA814676 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE198354_ebv_sampleRPM.csv.gz |
1.7 Kb |
(ftp)(http) |
CSV |
| GSE198354_hsa_sampleRPM.csv.gz |
37.8 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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