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| Status |
Public on Apr 24, 2023 |
| Title |
Integrated Transcriptome and Metabolome Identified Novel Candidate Genes Regulating Anthocyanin Biosynthesis in Colored-Grain Wheat (Triticum aestivum L.) |
| Organism |
Triticum aestivum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The present study aimed to investigate the anthocyanin components and identify relevant regulatory genes in purple wheat grain by carrying out transcriptome analyses. Methods: The seeds of purple grain wheat and white grain wheat were collected 30 days after flowering, and three biological replicates were set. Total RNA was isolated and purified using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's procedure. The RNA amount and purity of each sample was quantified using NanoDrop ND-1000. Then synthesizing the fragmented RNA into cDNA through the action of reverse transcriptase, and finally obtaining acDNA library. At last, we performed the 2×150bp paired-end sequencing (PE150) on an Illumina Novaseq™ 6000 following the vendor's recommended protocol. Results: A total of 10440 diferentially expressed genes were signifcantly enriched by RNA sequencing. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed signifcantly enriched flavonoid biosynthesis and anthocyanin biosynthesis in CW_S versus W_S. And the ANS and UFGT genes were predicted as core genes in anthocyanin biosynthesis. Conclusions: Our study represents the detailed analysis of wheat grain transcriptomes, with biologic replicates, generated by RNA-seq technology. Through this study, we speculated that ANS and UFGT genes are the core genes of anthocyanin biosynthesis.The significant differences of these genes affect the synthesis of anthocyanins in wheat grains, and thus affect the grain color of wheat.
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| Overall design |
The mRNA profiles of white grain wheat (W_S) and purple grain wheat (CW_S) grains 30 days after flowering.
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| Contributor(s) |
Liu X, Fang W |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 12, 2022 |
| Last update date |
Apr 24, 2023 |
| Contact name |
Liu Xiaoting |
| E-mail(s) |
t2775387002@139.com
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| Phone |
15649857980
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| Organization name |
Zhengzhou University
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| Street address |
100 Science Avenue
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| City |
Zhengzhou |
| ZIP/Postal code |
450001 |
| Country |
China |
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| Platforms (1) |
| GPL25409 |
Illumina NovaSeq 6000 (Triticum aestivum) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA815667 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE198515_genes_fpkm_expression.xlsx |
21.8 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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