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| Status |
Public on Mar 18, 2022 |
| Title |
Genetic and transcriptomic characteristics of RhlR-dependent quorum sensing in cystic fibrosis isolates of Pseudomonas aeruginosa: isolate E131 |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In people with the genetic disease cystic fibrosis (CF), bacterial infections involving the opportunistic pathogen Pseudomonas aeruginosa are a significant cause of morbidity and mortality. P. aeruginosa uses a cell-cell signaling mechanism called quorum sensing (QS) to regulate many virulence functions. One type of QS consists of acyl-homoserine lactone (AHL) signals produced by LuxI-type signal synthases, which bind a cognate LuxR-type transcription factor. In laboratory strains and conditions, P. aeruginosa employs two AHL synthase/receptor pairs arranged in a hierarchy, with the LasI/R system controlling the RhlI/R system and many downstream virulence factors. However, P. aeruginosa isolates with inactivating mutations in lasR are frequently isolated from chronic CF infections. We and others have shown that these isolates frequently use RhlR as the primary QS regulator. RhlR is rarely mutated in CF and environmental settings. We were interested if there were reproducible genetic characteristics of these isolates and if there was a central group of genes regulated by RhlR in all isolates. We examined five isolates and found signatures of adaptation common to CF isolates. Here, we analyzed CF clinial isolate E131. In this strain, RhlR positively regulates 105 genes, including those coding for known virulence factors. These results suggest a key group of QS-regulated factors important for pathogenesis of chronic infection, and position RhlR as a target for anti-QS therapeutics. Our work underscores the need to sample a diversity of isolates to understanding QS beyond what has been described in laboratory strains.
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| Overall design |
Examination of a rhlR-deletion mutant of a cystic fibrosis isolate of Pseudomonas aeruginosa vs. the isogenic parent strain
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| Contributor(s) |
Asfahl KL, Smalley NE, Chang AP, Dandekar AA |
| Citation(s) |
35471121 |
| |
| Submission date |
Mar 15, 2022 |
| Last update date |
May 05, 2022 |
| Contact name |
Kyle Lowe Asfahl |
| E-mail(s) |
kyleloweasfahl@gmail.com
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| Organization name |
University of Washington
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| Department |
Microbiology
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| Street address |
1705 NE Pacific St
|
| City |
Seattle |
| State/province |
Washington |
| ZIP/Postal code |
98195 |
| Country |
USA |
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| Platforms (1) |
| GPL24583 |
Illumina HiSeq 4000 (Pseudomonas aeruginosa) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA816521 |
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