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| Status |
Public on Jul 20, 2013 |
| Title |
Mechanistic information on perfluorooctanoic acid (PFOA) exposure in zebrafish (Danio rerio) |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
Perfluorooctanoic acid (PFOA) is one of the most used perfluorinated compounds in numerous applications and can be detected in environmental samples from around the globe. The aquatic environment is an important site for PFOA deposit. Nevertheless, the exact mode of action and its resulting toxicological effects on aquatic organisms remain largely unknown. To gain a more extensive understanding of the mode of action of teleost PFOA toxicity, transcriptomics, proteomics, biochemical parameters and reproduction were integrated in the present study. Male and female zebrafish were exposed to nominal concentrations of 0.1; 0.5 and 1 mg/l PFOA for 4 and 28 days resulting in an accumulation which was higher in males compared to females. These gender-related differences were likely caused by different elimination rates due to distinct hormone levels and differences in transport activity by solute carriers. The general mode of action of PFOA was believed to be an increase of the mitochondrial membrane permeability which caused effects on the electron transport system at the biochemical level and resulted in alterations of the oxidative phosphorylation, oxidative stress and apoptosis at the gene transcript and protein level. As a consequence, evidence for the replacement of the affected cells and organelles to sustain tissue homeostasis was found at the molecular level. The higher energy demand, due to these adverse effects, was provided by lowering the glycogen stores. Despite this increase in metabolic expenditure, no effects on reproduction were found indicating that the fish seemed to cope with exposure to the tested concentrations of PFOA.
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| Overall design |
Adult zebrafish (Danio rerio) were exposed to nominal concentrations of 0mg/l; 0.1mg/l; 1mg/l PFOA (perfluorooctanoic acid) for 28 days. Three different 25 litre aquaria per exposure concentration were used resulting in 3 biological replicates with each aquarium containing 8 male and 8 female zebrafish. The livers of 6 male fish and 6 female fish were pooled separately and snap frozen in liquid nitrogen. A reference sample was made by pooling equal amounts of RNA from all samples.
A carriage wheel design was used in which all samples were connected to the reference sample and the main contrasts of interest were made directly on the same microarrays as frequently as possible. This design resulted in technical triplicates of each sample.
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| Contributor(s) |
Hagenaars A, Vergauwen L, Benoot D, Laukens K, Knapen D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 15, 2010 |
| Last update date |
Jul 20, 2013 |
| Contact name |
An Hagenaars |
| E-mail(s) |
an.hagenaars@ua.ac.be
|
| Phone |
+32 (0)3 265 34 82
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| Fax |
+32 (0)3 265 34 97
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| URL |
http://www.ecotox.be/
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| Organization name |
University of Antwerp
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| Department |
Biology
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| Lab |
EB&T
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| Street address |
Groenenborgerlaan 171
|
| City |
Wilrijk |
| State/province |
Antwerp |
| ZIP/Postal code |
2020 |
| Country |
Belgium |
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| Platforms (1) |
| GPL9907 |
University of Antwerp Danio rerio oligo microarray_Agilent_15k_EBT |
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| Samples (24)
|
| GSM497143 |
[control_male_rep1]_[high_exposed_male_rep1] |
| GSM497144 |
[high exposed_male_rep2]_[high exposed_female_rep2] |
| GSM497278 |
[Reference]_[high exposed_female_rep2] |
| GSM497279 |
[high exposed_female_rep2]_[control_female_rep2] |
| GSM497280 |
[control_female_rep2]_[reference] |
| GSM497281 |
[control_female_rep2]_[low exposed_female_rep2] |
| GSM497288 |
[reference]_[low exposed_female_rep2] |
| GSM497289 |
[low exposed_female_rep2]_[high exposed_female_rep1] |
| GSM497290 |
[high exposed_female_rep1]_[reference] |
| GSM497291 |
[high exposed_female_rep1]_[control_female_rep1] |
| GSM497292 |
[reference]_[control_female_rep1] |
| GSM497302 |
[reference]_[control_male_rep1] |
| GSM497303 |
[control_female_rep1]_[low exposed_female_rep1] |
| GSM497304 |
[low exposed_female_rep1]_[reference] |
| GSM497305 |
[low exposed_female_rep1]_[low exposed_male_rep1] |
| GSM497306 |
[low exposed_male_rep1]_[reference] |
| GSM497307 |
[low exposed_male_rep1]_[control_male_rep1] |
| GSM497308 |
[high exposed_male_rep1]_[reference] |
| GSM497309 |
[high exposed_male_rep1]_[low exposed_male_rep2] |
| GSM497310 |
[reference]_[low exposed_male_rep2] |
| GSM497582 |
[low exposed_male_rep2]_[control_male_rep2] |
| GSM497583 |
[control_male_rep2]_[reference] |
| GSM497584 |
[control_male_rep2]_[high exposed_male_rep2] |
| GSM497585 |
[reference]_[high exposed_male_rep2] |
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| Relations |
| BioProject |
PRJNA121939 |
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