|
| Status |
Public on Jan 20, 2010 |
| Title |
Virus-derived siRNAs in Arabidopsis thaliana |
| Organisms |
Arabidopsis thaliana; Cucumber mosaic virus (strain FNY) |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We investigated the role of A. thaliana RDRs in the RNAi-mediated viral immunity by using a mutant of cucumber mosaic virus (CMV) that does not express the VSR protein 2b. CMV contains three positive-strand genomic RNAs and the 2b protein encoded by RNA2 is essential for infection by suppressing antiviral silencing initiated by either DCL4 or DCL2. Our results demonstrate an essential role for the amplification of viral siRNAs by either RDR1 or RDR6 in antiviral silencing. Further analyses, including Illumina sequencing of more than 3.5 million viral siRNAs, indicated target specificity of the two antiviral RDRs.
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| |
|
| Overall design |
Examination of the viral siRNAs of CMV in the wild-type and mutants of A. thaliana
|
| |
|
| Contributor(s) |
Wang X, Wu Q, Ding S |
| Citation(s) |
19966292 |
| |
| Submission date |
Jan 20, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Qingfa Wu |
| E-mail(s) |
qingfawu@ucr.edu
|
| Phone |
1-951-827-2340
|
| Organization name |
UC Riverside
|
| Lab |
Shou-Wei Ding
|
| Street address |
900 University Ave
|
| City |
Riverside |
| State/province |
CA |
| ZIP/Postal code |
92507 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9951 |
Illumina Genome Analyzer (Arabidopsis thaliana; Cucumber mosaic virus (strain FNY)) |
|
| Samples (6)
|
| GSM498721 |
CMV delta2b in Arabidopsis thaliana (col) |
| GSM498722 |
CMV delta2b in Arabidopsis thaliana (col), replicate |
| GSM498723 |
CMV delta2b in rdr1 mutant of Arabidopsis thaliana |
| GSM498724 |
CMV delta2b in rdr1 mutant of Arabidopsis thaliana, replicate |
| GSM498725 |
CMV delta2b in rdr1/2/6 mutant of Arabidopsis thaliana |
| GSM498726 |
CMV delta2b in rdr1/2/6 mutant of Arabidopsis thaliana, replicate |
|
| Relations |
| SRA |
SRP001740 |
| BioProject |
PRJNA122617 |
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