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| Status |
Public on May 02, 2023 |
| Title |
Liver transcriptomic and methylomic analyses identify transcriptional MAPK regulation in facultative hibernation of Syrian hamster [RNA-Seq] |
| Organism |
Mesocricetus auratus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Hibernation consist of alternating torpor/arousal phases, during which animals cope with repetitive hypothermia and ischemia-reperfusion. Due to limited transcriptomic and methylomic information for facultative hibernators, we here conducted RNA and whole genome bisulfite sequencing in liver of hibernating Syrian hamster (Mesocricetus auratus). Gene Ontology analysis was performed on 844 differentially expressed genes (DEGs) and confirmed the Liver, shift in metabolic fuel utilization, inhibition of RNA transcription and cell cycle regulation as found in seasonal hibernators. We Liver, show a so far unreported suppression of MAPK and PP1 pathways. Notably, hibernating hamsters Liver, showed upregulation of MAPK inhibitors (DUSPs and SPRYs) and reduced levels of MAPK induced transcription factors. Promoter methylation was found to modulate the expression of genes targeted by these transcription factors. In conclusion, we document gene regulation between hibernation phases, which may aid the identification of pathways and targets to prevent organ damage in transplantation or ischemia-reperfusion.
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| Overall design |
RNA Sequencing profiles for 9 Syrian Hamsters in the following hibernation stages: euthermic, torpor late and arousal early.
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| Contributor(s) |
Oosterhof MM, Coussement L, Guryev V, Reitsema VA, Bruintjes JJ, Goris M, Bouma HR, De Meyer T, Rots MG, Henning RH |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Mar 30, 2022 |
| Last update date |
May 03, 2023 |
| Contact name |
Jeroen Galle |
| Organization name |
Ghent University
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| Department |
Dept. Of Mathematical Modelling, Statistics and Bioinformatics
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| Lab |
Biobix, Lab of Bioinformatics and Computational Genomics
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| Street address |
Coupure Links 653
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| City |
Ghent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL24490 |
Illumina NextSeq 500 (Mesocricetus auratus) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE199817 |
Liver transcriptomic and methylomic analyses identify transcriptional MAPK regulation in facultative hibernation of Syrian hamster |
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| Relations |
| BioProject |
PRJNA821536 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE199814_20190603_MesAur_readcounts.txt.gz |
281.1 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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