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| Status |
Public on Dec 22, 2022 |
| Title |
A bioengineered tridimensional substrate designed for mesenchymal stem cells culture: whole transcriptomic analysis |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this work we aimed to evaluate how the three-dimensionality of an engineered scaffold, concieved to emulate the stem cell niche, can influence the whole gene expression profile of mesenchymal stem cells. We performed a differential expression analysis, comparing the transcriptome of MSCs grown in 3D Nichoids with that of MSCs grown on 2D Nichoids, a structure with the same grid geometry, except for the overall tickness which allows a spreading expansion of cells. We found a total of 603 differentially expressed genes that were used also for functional enrichment analysis.
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| Overall design |
Total RNA extracted from rBMSCs cultured on 3D Nichoid (n=3) and 2D Nichoid (n=3) after 4 days of culture
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| Contributor(s) |
Testa C, Oliveto S, Jacchetti E, Donnaloja F, Martinelli C, Pinoli P, Osellame R, Cerullo G, Ceri S, Biffo S, Raimondi MT |
| Citation(s) |
36686258 |
| Submission date |
Mar 31, 2022 |
| Last update date |
Feb 06, 2023 |
| Contact name |
Carolina Testa |
| E-mail(s) |
carolina.testa@polimi.it
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| Organization name |
Politecnico di Milano
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| Street address |
Piazza Leonardo da Vinci 32
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| City |
Milano |
| ZIP/Postal code |
20133 |
| Country |
Italy |
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| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA821697 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE199846_RAW.tar |
760.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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