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Series GSE199933 Query DataSets for GSE199933
Status Public on Apr 04, 2022
Title Single-cell RNA-seq of goat early embyos and GFFs cells
Organism Capra hircus
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary We use high-throughput single cell RNA-seq to determine expression profile of IVF-2cell 、 IVF-8cell and SCNT-8cell embryos,and C1 and over3720. At present, the strand-specific library could not be generated for the analysis of goat embryo lncRNA because of the lack of available specific rRNA removal kits for goats. In this study, single-cell RNA-seq technology was used to analyze early embryonic transcripts, enrich the involving transcripts, and construct an common transcript library. For identifying transcripts expressed during the early goat embryonic stage, three cDNA libraries were established from early goat embryos at the three developmental stages, namely, two-cell (IVF-2c)、 eight-cell embryos (IVF-8c) and somatic cell nuclear transfer(SCNT-8c). The GFFs cells transfected with control (no-load) and recombinant vectors were collected, total RNA was extracted, and the quality of the RNA was determined.
 
Overall design Examination of expression differences between goat IVF 2-cell 、IVF 8-cell emryos and SCNT 8-cell
 
Contributor(s) Liu J, Jin M, Zhao L
Citation(s) 37490350
Submission date Apr 01, 2022
Last update date Sep 21, 2023
Contact name Miaomiao Jin
E-mail(s) liujun2013@nwsuaf.edu.cn
Phone 18437958771
Organization name northwest A&F university
Street address yangling
City xian
ZIP/Postal code 712100
Country China
 
Platforms (1)
GPL19149 Illumina HiSeq 2500 (Capra hircus)
Samples (9)
GSM5994255 IVF_2c_RNAseq
GSM5994256 IVF_8c_RNAseq
GSM5994257 SCNT_8c_RNAseq
Relations
BioProject PRJNA822291

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE199933_RAW.tar 3.1 Mb (http)(custom) TAR (of XLSX)
GSE199933_fpkm.txt.gz 988.8 Kb (ftp)(http) TXT
GSE199933_lncRNA.fasta.gz 6.2 Mb (ftp)(http) FASTA
GSE199933_lncRNA_FPKM.txt.gz 415.9 Kb (ftp)(http) TXT
GSE199933_mRNA_FPKM.txt.gz 396.6 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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