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| Status |
Public on Mar 17, 2023 |
| Title |
Pleomorphic Variants of Borreliella (syn. Borrelia) burgdorferi Express Evolutionary Distinct Transcriptomes |
| Organism |
Borreliella burgdorferi |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The objective of the study was to uncover the transcriptional differences between B. burgdorferi pleomorphic forms (spirochetes, round bodies, blebs and biofilms).
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| Overall design |
Spirochetes were raised in BSK-H medium containing 6% rabbit serum in conical tubes with a tightly closed lid. Round bodies were gained by resuspending and incubating spirochetes in molecular-biology grade water for 10-30 minutes. Blebs were raised by adding spirochetes into conical tubes with a vented lid. Biofilms were induced by cultivation of spirochetes in tissue-culture dishes. Each sample was represented in three replicates, except the bleb samples which were represented in two replicates.
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| Contributor(s) |
Čorak N, Anniko S, Daschkin-Steinborn C, Krey V, Koska S, Futo M, Široki T, Woichansky I, Opašić L, Kifer D, Tušar A, Maxeiner H, Domazet-Lošo M, Nicolaus C, Domazet-Lošo T |
| Citation(s) |
36982667 |
| |
| Submission date |
Apr 01, 2022 |
| Last update date |
Apr 04, 2023 |
| Contact name |
Tomislav Domazet-Loso |
| E-mail(s) |
tdomazet@irb.hr
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| Organization name |
Institut Rudjer Bošković
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| Department |
Division of Molecular Biology
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| Lab |
Laboratory of Evolutionary Genetics
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| Street address |
Bijenička cesta 54
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| City |
Zagreb |
| State/province |
Grad Zagreb |
| ZIP/Postal code |
10000 |
| Country |
Croatia |
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| Platforms (1) |
| GPL24990 |
Illumina NextSeq 500 (Borreliella burgdorferi) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA822307 |
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