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Series GSE200271 Query DataSets for GSE200271
Status Public on Dec 25, 2023
Title RNA-sequencing of estradiol inducible DOF6 overexpression (DOF6ioex) and rgl2-13 DOF6ioex seeds imbibed in mock or 50µM estradiol for 8h
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary The DELLA protein RGA-LIKE2 (RGL2) is a key transcriptional repressor of gibberellic acid (GA) signaling that regulates seed germination.RGL2 lacks a DNA binding domain and requires a transcription factor to induce GATA12 expression. Our data show that this RGL2-containing protein complex includes DNA BINDING1 ZINC FINGER6 (DOF6), which is a known negative regulator of germination in freshly harvested seeds. The RGL2-DOF6 complex regulates primary seed dormancy in Arabidopsis by regulating downstreming targets.
 
Overall design Differential gene expression in freshly harvested DOF6ioex and rgl2-13 DOF6ioex seeds imbibed in mock or 50µM estradiol at room temperature for 8h
 
Contributor(s) Ravindran P, Krishnamurthy P, Ney CS, Kumar PP
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Submission date Apr 06, 2022
Last update date Dec 25, 2023
Contact name Prakash P Kumar
E-mail(s) dbskumar@nus.edu.sg
Phone 65162859
Organization name NUS
Department DBS
Street address 14 Science Drive 4
City Singapore
State/province Singapore
ZIP/Postal code 117556
Country Singapore
 
Platforms (1)
GPL24025 BGISEQ-500 (Arabidopsis thaliana)
Samples (9)
GSM6025837 DOF6ioex_Mock1
GSM6025838 DOF6ioex_Mock2
GSM6025839 DOF6ioex_Mock3
Relations
BioProject PRJNA823917

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE200271_DOF6ioex_estradiol_Vs_rgl2-13DOF6ioex_estradiol.xlsx 7.3 Mb (ftp)(http) XLSX
GSE200271_DOF6ioex_mock_Vs_DOF6ioex_estradiol.xlsx 7.2 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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