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| Status |
Public on Apr 13, 2022 |
| Title |
Concentrations of progesterone prior to luteolysis modulates the uterine luminal transcriptome in the subsequent cycle in cattle |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Sex-steroids concentrations throughout the estrous cycle modulate endometrial function and fertility in cattle. The objective of this study was to compare the post-estrus endometrial transcriptome of cows that were exposed to contrasting pre-estrus concentrations of progesterone before they displayed estrus and ovulated spontaneously. We hypothesized that greater pre-estrus concentration of progesterone alters post-estrus endometrial function even after the display of estrus and spontaneous ovulation.
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| Overall design |
Bos indicus-influenced cross-bred beef cows exposed to either high (H) or low (L) pre-estrus concentrations of progesterone. On day -9, the cows assigned to the high progesterone treatment received a new CIDR and 2 mL GnRH i.m. while the cows assigned to low progesterone treatment received a CIDR used twice previously, 5 mL PGF2α i.m. and 2 mL GnRH i.m. Ovarian ultrasonography and blood collections were performed on d -9, d -2, d -0.5, d 0 (observed estrus), d 4, d 7, and d 14 for measurement of ovarian structures, progesterone, and estradiol. Luminal epithelial cells were collected using a cytology brush on d 4, d 7, and d 14 for RNA-sequencing analysis.
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| Contributor(s) |
Binelli M, Peñagaricano F |
| Citation(s) |
36930057, 37224090 |
| Submission date |
Apr 07, 2022 |
| Last update date |
Jun 22, 2023 |
| Contact name |
Francisco Peñagaricano |
| E-mail(s) |
fpenagarican@wisc.edu
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| Organization name |
University of Wisconsin-Madison
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| Department |
Animal and Dairy Sciences
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| Street address |
1675 Observatory Drive
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| City |
Madison |
| State/province |
Wisconsin |
| ZIP/Postal code |
53706 |
| Country |
USA |
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| Platforms (1) |
| GPL26012 |
Illumina NovaSeq 6000 (Bos taurus) |
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| Samples (89)
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| GSM6032043 |
Endometrium, r-5 |
| GSM6032044 |
Endometrium, r-6 |
| GSM6032045 |
Endometrium, r-7 |
| GSM6032046 |
Endometrium, r-8 |
| GSM6032047 |
Endometrium, r-9 |
| GSM6032048 |
Endometrium, r-10 |
| GSM6032049 |
Endometrium, r-11 |
| GSM6032050 |
Endometrium, r-12 |
| GSM6032051 |
Endometrium, r-13 |
| GSM6032052 |
Endometrium, r-14 |
| GSM6032053 |
Endometrium, r-15 |
| GSM6032054 |
Endometrium, r-16 |
| GSM6032055 |
Endometrium, r-17 |
| GSM6032056 |
Endometrium, r-18 |
| GSM6032057 |
Endometrium, r-19 |
| GSM6032058 |
Endometrium, r-20 |
| GSM6032059 |
Endometrium, r-21 |
| GSM6032060 |
Endometrium, r-23 |
| GSM6032061 |
Endometrium, r-24 |
| GSM6032062 |
Endometrium, r-25 |
| GSM6032063 |
Endometrium, r-27 |
| GSM6032064 |
Endometrium, r-28 |
| GSM6032065 |
Endometrium, r-29 |
| GSM6032066 |
Endometrium, r-31 |
| GSM6032067 |
Endometrium, r-32 |
| GSM6032068 |
Endometrium, r-33 |
| GSM6032069 |
Endometrium, r-34 |
| GSM6032070 |
Endometrium, r-35 |
| GSM6032071 |
Endometrium, r-36 |
| GSM6032072 |
Endometrium, r-37 |
| GSM6032073 |
Endometrium, r-38 |
| GSM6032074 |
Endometrium, r-39 |
| GSM6032075 |
Endometrium, r-40 |
| GSM6032076 |
Endometrium, r-41 |
| GSM6032077 |
Endometrium, r-42 |
| GSM6032078 |
Endometrium, r-43 |
| GSM6032079 |
Endometrium, r-44 |
| GSM6032080 |
Endometrium, r-45 |
| GSM6032081 |
Endometrium, r-46 |
| GSM6032082 |
Endometrium, r-47 |
| GSM6032083 |
Endometrium, r-48 |
| GSM6032084 |
Endometrium, r-49 |
| GSM6032085 |
Endometrium, r-50 |
| GSM6032086 |
Endometrium, r-52 |
| GSM6032087 |
Endometrium, r-53 |
| GSM6032088 |
Endometrium, r-55 |
| GSM6032089 |
Endometrium, r-57 |
| GSM6032090 |
Endometrium, r-58 |
| GSM6032091 |
Endometrium, r-59 |
| GSM6032092 |
Endometrium, r-60 |
| GSM6032093 |
Endometrium, r-62 |
| GSM6032094 |
Endometrium, r-63 |
| GSM6032095 |
Endometrium, r-64 |
| GSM6032096 |
Endometrium, r-65 |
| GSM6032097 |
Endometrium, r-67 |
| GSM6032098 |
Endometrium, r-68 |
| GSM6032099 |
Endometrium, r-69 |
| GSM6032100 |
Endometrium, r-70 |
| GSM6032101 |
Endometrium, r-71 |
| GSM6032102 |
Endometrium, r-72 |
| GSM6032103 |
Endometrium, r-73 |
| GSM6032104 |
Endometrium, r-74 |
| GSM6032105 |
Endometrium, r-75 |
| GSM6032106 |
Endometrium, r-76 |
| GSM6032107 |
Endometrium, r-77 |
| GSM6032108 |
Endometrium, r-78 |
| GSM6032109 |
Endometrium, r-80 |
| GSM6032110 |
Endometrium, r-82 |
| GSM6032111 |
Endometrium, r-83 |
| GSM6032112 |
Endometrium, r-84 |
| GSM6032113 |
Endometrium, r-86 |
| GSM6032114 |
Endometrium, r-87 |
| GSM6032115 |
Endometrium, r-90 |
| GSM6032116 |
Endometrium, r-93 |
| GSM6032117 |
Endometrium, r-95 |
| GSM6032118 |
Endometrium, r-96 |
| GSM6032119 |
Endometrium, r-98 |
| GSM6032120 |
Endometrium, r-99 |
| GSM6032121 |
Endometrium, r-100 |
| GSM6032122 |
Endometrium, r-101 |
| GSM6032123 |
Endometrium, r-102 |
| GSM6032124 |
Endometrium, r-104 |
| GSM6032125 |
Endometrium, r-105 |
| GSM6032126 |
Endometrium, r-108 |
| GSM6032127 |
Endometrium, r-112 |
| GSM6032128 |
Endometrium, r-113 |
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| Relations |
| BioProject |
PRJNA824318 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE200361_RAW.tar |
9.7 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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