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| Status |
Public on Apr 17, 2022 |
| Title |
Specific targeting of plasmids with Argonaute enables genome editing |
| Organism |
Escherichia coli |
| Experiment type |
Other
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| Summary |
Prokaryotic Argonaute nuclease induces DNA interference between plasmid and chromosomal DNA to promote genome recombineering
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| Overall design |
Argonaute-associated smDNA from E.coli transformed by plasmids of different constructions was analysed to elucidate the requirements for genomic DNA cleavage by CbAgo during DNA interference and integration of plasmid-encoded sequences into the homologous chromosomal locus
Second part of data on DNA interference research. Additional libraries from 2 clones were prepared to show the integration of plasmid-encoded genes into the homologous chromosomal locus promoted by bacterial Argonaut protein
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| Citation(s) |
36987855 |
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| Submission date |
Apr 12, 2022 |
| Last update date |
Mar 30, 2023 |
| Contact name |
Anastasiia Okhtienko |
| Organization name |
Institute of Molecular Genetics, Russian Academy of Sciences
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| Lab |
RNA Biology and Epigenetics
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| Street address |
Akademika Kurchatova square 2
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| City |
Moscow |
| ZIP/Postal code |
123182 |
| Country |
Russia |
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| Platforms (1) |
| GPL18133 |
Illumina HiSeq 2500 (Escherichia coli) |
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| Samples (15)
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| GSM6042377 |
pBAD_CbAgo_lacI_0.2, 200 bp |
| GSM6042378 |
pBAD_CbAgo_lacI_0.3, 300 bp |
| GSM6042379 |
pBAD_CbAgo_lacI_0.45, 450 bp |
| GSM6042380 |
pBAD_CbAgo_lacI_0.6, 600 bp |
| GSM6042381 |
pBAD_CbAgo_lacI_full, full gene (1083 bp) |
| GSM6042382 |
pBAD_CbAgo_lacI_rec, recoded gene |
| GSM6042383 |
pBAD_yffP300_noCS, 300 bp insert, no SceI site |
| GSM6042384 |
pBAD_yffP300_mutCS, 300 bp insert, mut SceI site |
| GSM6042385 |
pBAD_yffP1000_mutCS, 1000 bp insert, mut SceI site |
| GSM6997580 |
MG1655_CmR_SpS_clone1 |
| GSM6997581 |
MG1655_CmR_SpS_clone2 |
| GSM6997582 |
MG1655_CmR_sum |
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| Relations |
| BioProject |
PRJNA826095 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE200694_RAW.tar |
1.4 Mb |
(http)(custom) |
TAR (of TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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