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| Status |
Public on Sep 20, 2022 |
| Title |
Isolation of ferret astrocytes reveals their morphological, transcriptional, and functional differences from mouse astrocytes |
| Organisms |
Mustela putorius furo; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To identify differently expressed genes between ferret and mouse astrocytes, we performed RNA-seq studies on primary ferret and mouse astrocytes cultured in serum free medium. We isolated primary astrocytes from mixed glial cultures which are prepared from the cortices of ferrets and mice at postnatal day 1. Each biological replicate was generated from 2 individuals of the same species. We detected substantial changes in the expression of genes associated with cell proliferation and migration, and identified ferret astrocyte-specific genes.
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| Overall design |
We generated mRNA profiles of primary astrocytes which were prepared from ferret and mouse neonatal cortices using Illumina HiSeq 2000 platform.
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| Contributor(s) |
Roboon J, Hattori T, Kannon T, Hosomichi K, Tajima A, Hori O |
| Citation(s) |
36274991 |
| Submission date |
Apr 21, 2022 |
| Last update date |
Oct 31, 2022 |
| Contact name |
Tsuyoshi Hattori |
| E-mail(s) |
thattori@staff.kanazawa-u.ac.jp
|
| Phone |
0762652162
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| Organization name |
Kanazawa University
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| Department |
Department of Neuroanatomy
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| Street address |
13-1 Takara-Machi
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| City |
Kanazawa City |
| State/province |
Ishikawa |
| ZIP/Postal code |
920-8640 |
| Country |
Japan |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL24172 |
Illumina HiSeq 2000 (Mustela putorius furo) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA830345 |
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