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Series GSE20120 Query DataSets for GSE20120
Status Public on Apr 09, 2012
Title Identification of miRNAs involved in cell response to ionising radiation and modeled microgravity
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary BACKGROUND:
Ionizing radiation (IR) can be extremely harmful for human cells since an improper DNA-damage response (DDR) to IR can contribute to carcinogenesis initiation. Perturbations in DDR pathway can originate from alteration in the functionality of the microRNA-mediated gene regulation, being microRNAs (miRNAs) small noncoding RNA that act as post-transcriptional regulators of gene expression. In this study we gained insight into the role of miRNAs in the regulation of DDR to IR under microgravity, a condition of weightlessness experienced by astronauts during space missions, which could have a synergistic action on cells, increasing the risk of radiation exposure.
METHODOLOGY/PRINCIPAL FINDINGS:
We analyzed miRNA expression profile of human peripheral blood lymphocytes (PBL) incubated for 4 and 24 h in normal gravity (1 g) and in modeled microgravity (MMG) during the repair time after irradiation with 0.2 and 2Gy of γ-rays. Our results show that MMG alters miRNA expression signature of irradiated PBL by decreasing the number of radio-responsive miRNAs. Moreover, let-7i*, miR-7, miR-7-1*, miR-27a, miR-144, miR-200a, miR-598, miR-650 are deregulated by the combined action of radiation and MMG. Integrated analyses of miRNA and mRNA expression profiles, carried out on PBL of the same donors, identified significant miRNA-mRNA anti-correlations of DDR pathway. Gene Ontology analysis reports that the biological category of "Response to DNA damage" is enriched when PBL are incubated in 1 g but not in MMG. Moreover, some anti-correlated genes of p53-pathway show a different expression level between 1 g and MMG. Functional validation assays using luciferase reporter constructs confirmed miRNA-mRNA interactions derived from target prediction analyses.
CONCLUSIONS/SIGNIFICANCE:
On the whole, by integrating the transcriptome and microRNome, we provide evidence that modeled microgravity can affects the DNA-damage response to IR in human PBL.
 
Overall design miRNA expression profiling was analysed in human peripheral blood lymphocytes (PBL) irradiated with gamma-rays (0.2 and 2.0 Gy) and incubated in modeled microgravity (mmg) and in parallel ground conditions (1g) for 4h and 24h. Six independent experiments were performed for each donor to address wich miRNAs were regulated on IR stress. The level of each miRNA was represented as Log2.
 
Contributor(s) Girardi C, De Pitta C, Casara S, Sales G, Lanfranchi G, Celotti L, Mognato M
Citation(s)
  • Girardi C, De Pittà C, Casara S, Sales G et al. Analysis of miRNA and mRNA expression profiles highlights alterations in ionizing radiation response of human lymphocytes under modeled microgravity. PLoS One 2012;7(2):e31293. PMID: 22347458
Submission date Feb 01, 2010
Last update date May 07, 2014
Contact name Gerolamo Lanfranchi
E-mail(s) stefano.cagnin@unipd.it
Phone +39-0498276219
Organization name University of Padova
Department CRIBI - Biotechnology Center and Biology Department
Lab Functional Genomics Lab
Street address Via U. Bassi, 58/B
City Padova
ZIP/Postal code 35131
Country Italy
 
Platforms (1)
GPL8227 Agilent-019118 Human miRNA Microarray 2.0 G4470B (miRNA ID version)
Samples (72)
GSM502089 4h-1g-P
GSM502090 4h-mmg-P
GSM502091 24h-1g-P
Relations
BioProject PRJNA124259

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20120_RAW.tar 1.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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