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Series GSE201208 Query DataSets for GSE201208
Status Public on Oct 12, 2022
Title Genome-wide screen reveals UL49 is an essential vPIC subunit that transcribes late Human Cytomegalovirus genes
Organisms Homo sapiens; Human betaherpesvirus 5
Experiment type Expression profiling by high throughput sequencing
Summary More than half the world's population is infected with Human Cytomegalovirus (HCMV), increasing risks for the immuno-compromised, and causing significant congenital birth defects. The HCMV genome encodes >170 proteins; many that remain uncharacterised, which restricts development opportunities for new antivirals. In this study, a molecular assay was developed to conduct a genome-wide HCMV screen to identify genes that function during late stages of infection. Saliently, loss of UL49 completely abolished production of infectious virions. During infection, UL49 displayed leaky late expression kinetics and nuclear localization. Functionally, UL49 is as an essential subunit of the viral pre-initiation complex (vPIC), enhancing transcription of late viral genes containing non-canonical TATT motif promoters. RNA-seq revealed the full repertoire of vPIC-regulated genes, including transcripts coding for capsid subunits, envelope glycoproteins and egress-associated tegument proteins. Therefore, UL49 together with other vPIC subunits, serve as fundamental HCMV effectors that govern successful completion of the replication cycle.
 
Overall design Comparative gene expression profiling analysis of RNA-seq data for HCMV AD169 WT and vPIC subunit KO infected MRC5 fibroblasts at 72 HPI.
 
Contributor(s) Turner DL, Barugahare AA, Russ BE, Turner SJ, Mathias RA
Citation(s) 36204275
Submission date Apr 21, 2022
Last update date Oct 12, 2022
Contact name Monash Bioinformatics Platform
Organization name Monash Bioinformatics Platform
Lab Monash Bioinformatics Platform
Street address Wellington Road
City Clayton
State/province VIC
ZIP/Postal code 3800
Country Australia
 
Platforms (1)
GPL32186 MGISEQ-2000RS (Homo sapiens; Human betaherpesvirus 5)
Samples (17)
GSM6052708 HCMV AD169 infected MRC5, WT virus, MOI = 3, 72 HPI, rep 1
GSM6052709 HCMV AD169 infected MRC5, WT virus, MOI = 3, 72 HPI, rep 2
GSM6052710 HCMV AD169 infected MRC5, WT virus, MOI = 3, 72 HPI, rep 3
Relations
BioProject PRJNA830359

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Supplementary file Size Download File type/resource
GSE201208_NonStrandedCounts-withNames_hcmv_human.txt.gz 1.3 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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