 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 01, 2022 |
| Title |
Acinetobacter baumannii response to cefiderocol challenge in human urine |
| Organism |
Acinetobacter baumannii |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Cefiderocol (CFDC) is a novel chlorocatechol-substituted siderophore approved to treat complicated urinary tract infections and for hospital-acquired and ventilator-acquired pneumonia. In previous work, human fluids, were shown to increase the minimum inhibitory concentration (MICs) of Acinetobacter baumannii against CFDC and reduce the expression of genes related to iron uptake systems, which could explain the need for higher concentrations of CFDC to exert inhibitory action. Herein, we analyzed the impact of human urine (HU), which contains low albumin concentrations, on the expression of iron-uptake related genes and MIC values of two carbapenem-resistant A. baumannii. Levels of resistance to CFDC were not modified by HU in strain AMA40 but were reduced in the case of strain AB5075. Testing other carbapenem-resistant A. baumannii isolates showed that the CFDC MICs were unmodified or reduced in the presence of HU. The expression of piuA, pirA, bauA, and bfnH determined by qRT-PCR was enhanced in both strains when HU was present in the culture medium. All four tested genes are involved in recognizing ferric siderophore complexes or internalization into the cell’s cytosol. In contrast, the effect of HU on genes associated with resistance to β-lactams, antibiotics commonly used to treat urinary tract infections caused by A. baumannii, was variable; the transcriptional analysis of pbp1, pbp3, blaOXA-51-like, blaADC, and blaNDM-1 showed significant variation. In summary, HU, probably due to the albumin and free iron content, does not adversely impact or slightly improves the activity of CFDC when tested against A. baumannii in urine in contrast to other human bodily fluids.
|
| |
|
| Overall design |
Total mRNA of Acinetobacter baumanii AB5075 or Acinetobacter baumannii AMA40 was isolated from bacteria incubated in Mueller Hinton medium with or without 50% human urine
|
| |
|
| Contributor(s) |
Ramirez MS, Sieira R |
| Citation(s) |
35610334, 38853891, 39160175 |
| |
| Submission date |
Apr 21, 2022 |
| Last update date |
Sep 27, 2024 |
| Contact name |
M. S. Ramirez |
| Organization name |
California State University Fullerton
|
| Street address |
800 N. State College Blvd
|
| City |
Fullerton |
| State/province |
CA |
| ZIP/Postal code |
DBH-117A |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL28641 |
Illumina NovaSeq 6000 (Acinetobacter baumannii) |
|
| Samples (12)
|
| GSM6056849 |
AB5075 Mueller Hinton 50% Urine replicate 1 |
| GSM6056850 |
AB5075 Mueller Hinton 50% Urine replicate 2 |
| GSM6056851 |
AB5075 Mueller Hinton 50% Urine replicate 3 |
|
| Relations |
| BioProject |
PRJNA830513 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE201259_RAW.tar |
690.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...