 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 10, 2022 |
| Title |
Gene expression in COPD and non-diseased nasopharyngeal organoids following Pseudomonas aeruginosa infection |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Pseudomonas aeruginosa is a common bacteria leading to exacerbations of chronic obstructive pulmonary disease (COPD) patients while this bacteria can be easily eradicated by the immune systems of healthy individuals. Human airway organoids derived from healthy individuals and COPD patients were infected with pseudomonas aeruginosa. This project aims (1) to understand the differences in gene expressions in healthy and COPD airway organoids during stable condition, without infection and (2) to investigate differential pathogenic mechanism (i.e. antimicrobial defense) of pseudomonoas aeruginosa infection in healthy and COPD populations. Three healthy donors and three COPD patients were included in this study and samples were collected with and without pseudomonas aeruginosa infection.
|
| |
|
| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for COPD and non-diseased nasopharyngeal organoids, with or without infection of Pseudomonas aeruginosa
|
| |
|
| Contributor(s) |
Chan LY, Ivan FX, Cheng HS, Tan NS, Chotirmall SH |
| Citation(s) |
36496442, 37963864 |
| |
| Submission date |
Apr 25, 2022 |
| Last update date |
Dec 06, 2023 |
| Contact name |
Fransiskus Xaverius Ivan |
| E-mail(s) |
fivan@ntu.edu.sg
|
| Organization name |
Nanyang Technological University
|
| Department |
Lee Kong Chian School of Medicine
|
| Street address |
11 Mandalay Road
|
| City |
Singapore |
| ZIP/Postal code |
308232 |
| Country |
Singapore |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (12)
|
| GSM6064745 |
COPD nasopharyngal organoids, No infection, 6h post infection NRMK4 |
| GSM6064746 |
COPD nasopharyngal organoids, No infection, 6h post infection NRMK5 |
| GSM6064747 |
COPD nasopharyngal organoids, No infection, 6h post infection NRMK6 |
| GSM6064748 |
Non-diseased nasopharyngal organoids, No infection, 6h post infection NRMK10 |
| GSM6064749 |
Non-diseased nasopharyngal organoids, No infection, 6h post infection NRMK11 |
| GSM6064750 |
Non-diseased nasopharyngal organoids, No infection, 6h post infection NRMK12 |
| GSM6064751 |
COPD nasopharyngal organoids, Infection with P. aeruginosa, 6h post infection NRPA4 |
| GSM6064752 |
COPD nasopharyngal organoids, Infection with P. aeruginosa, 6h post infection NRPA5 |
| GSM6064753 |
COPD nasopharyngal organoids, Infection with P. aeruginosa, 6h post infection NRPA6 |
| GSM6064754 |
Non-diseased nasopharyngal organoids, Infection with P. aeruginosa, 6h post infection NRPA10 |
| GSM6064755 |
Non-diseased nasopharyngal organoids, Infection with P. aeruginosa, 6h post infection NRPA11 |
| GSM6064756 |
Non-diseased nasopharyngal organoids, Infection with P. aeruginosa, 6h post infection NRPA12 |
|
| Relations |
| BioProject |
PRJNA831839 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE201465_RAW.tar |
3.2 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...