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| Status |
Public on Feb 26, 2024 |
| Title |
RNA-sequencing to idendify axon RNAs in dentate gyrus granule cells, dorsal root ganglion cells and retinal ganglion cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To analyze axon transcriptome of dentate gyrus granule cells, dorsal root ganglion cells and retinal ganglion cells, we perfomed axon RNA sequencing.Neurons were cultured in microfluidic chambers and axons were isolated. Total RNA of axons was collected separately and subjected to library construction using M01440v2 NuGEN Trio RNA-seq kit. After sequencencing,we used TPM method to normalize RNA-seq reads.
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| Overall design |
Three axon samples from different cells as experimental group.
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| Contributor(s) |
Yang Z, Yu J, Ji S |
| Citation(s) |
37067243 |
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| Submission date |
Apr 26, 2022 |
| Last update date |
Feb 26, 2024 |
| Contact name |
Zhuoxuan Yang |
| E-mail(s) |
12032900@mail.sustech.edu.cn
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| Phone |
17604514873
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| Organization name |
Southern University of Science and Technology
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| Street address |
No. 1088 Xueyuan Avenue, Nanshan District
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| City |
Shenzhen |
| State/province |
Guangdong |
| ZIP/Postal code |
518055 |
| Country |
China |
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| Platforms (1) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA832213 |
