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| Status |
Public on Jan 09, 2023 |
| Title |
Expression data from C. elegans harboring type 1-like and type 2-like calreticulin mutations of MPN patients |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
There is growing evidence that Ph-negative myeloproliferative neoplasms (MPNs) are blood cancers in which multiple molecular mechanisms are significantly disturbed. Since their discovery in 2016, CALR (calreticulin) type 1 and type 2 driver mutations have been demonstrated to trigger pathogenic mechanisms apart from the well-documented activation of JAK2/MPL-related pathways affecting these diseases. C. elegans seems to be a suitable model for the study of these mechanisms since they have a CALR ortholog (crt-1) but have no JAK or MPL ortholog.
We used microarrays in C. elegans strains harboring patient-like calreticulin mutations to identify JAK/MPL-independent molecular mechanisms derived from mutant calreticulin and identified a significant disruption of some of the typically altered biological processes in cancer and Ph-negative MPN patients. Finally, we compared the expression of the genes participating in these processes with a calreticulin knockout strain in order to examine if the alteration of these processes is a consequence of a gain or a loss of function of the mutant protein.
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| Overall design |
C. elegans calreticulin-mutated strains and the wild-type strain (N2 Bristol) were synchronized and selected at the late L4 developmental stage for RNA extraction and hybridization of Affymetrix microarrays. Three samples per strain were analyzed in all cases, except for type 1 mutants and the knockout strain.
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| Contributor(s) |
Guijarro-Hernández A, Eder-Azanza L, Hurtado C, Vizmanos JL |
| Citation(s) |
36611979 |
| Submission date |
Apr 26, 2022 |
| Last update date |
Jan 12, 2023 |
| Contact name |
José Luis Vizmanos |
| E-mail(s) |
jlvizmanos@unav.es
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| Organization name |
University of Navarra
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| Department |
Department of Biochemistry and Genetics
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| Street address |
Irunlarrea, 1
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| City |
Pamplona |
| State/province |
Navarra |
| ZIP/Postal code |
31008 |
| Country |
Spain |
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| Platforms (1) |
| GPL19230 |
[EleGene-1_0-st] Affymetrix C. elegans Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (10)
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| GSM6068300 |
C. elegans CRT-1(mut1), biological rep1 |
| GSM6068301 |
C. elegans CRT-1(mut1), biological rep2 |
| GSM6068302 |
C. elegans CRT-1(mut2), biological rep1 |
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| Relations |
| BioProject |
PRJNA832284 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE201599_RAW.tar |
60.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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