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| Status |
Public on Oct 19, 2010 |
| Title |
Expression data from white and brown adipose tissue (WAT and BAT) of per2-/- and control mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We found that the circadian protein PER2 interacts with the nuclear receptor PPARgamma to repress its activity. PPARgamma is a master regulator of adipogenesis and lipid metabolism and is very abundant in adipose tissue. We used microarrays to detail the global program of gene expression in adipose tissue lacking the per2 gene. This analysis identified several PPARgamma target genes up-regulated in adipose tissue from per2-/- mice.
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| Overall design |
Per2-/- and per2+/+ male mice (Bae et al., 2001) were housed under 12 hr light/12 hr dark (LD) cycles. Mice 20 weeks old were sacrificed at the same time and adipose tissue (WAT and BAT) was collected for RNA extraction. 3 biological replicates per mouse/tissue.
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| Contributor(s) |
Grimaldi B, Sassone-Corsi P |
| Citation(s) |
21035761 |
| Submission date |
Feb 02, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
benedetto grimaldi |
| E-mail(s) |
bgrimald@uci.edu
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| Phone |
949 824 8056
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| Organization name |
university of california, irvine
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| Department |
pharmacology
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| Lab |
Sassone-Corsi
|
| Street address |
2226B Gillespie NRF
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92697 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| GSM506024 |
WAT from per2+/+ mice, biological rep1 |
| GSM506025 |
WAT from per2+/+ mice, biological rep2 |
| GSM506026 |
WAT from per2+/+ mice, biological rep3 |
| GSM506027 |
WAT from per2-/- mice, biological rep1 |
| GSM506028 |
WAT from per2-/- mice, biological rep2 |
| GSM506029 |
WAT from per2-/- mice, biological rep3 |
| GSM506030 |
BAT from per2+/+ mice, biological rep1 |
| GSM506031 |
BAT from per2+/+ mice, biological rep2 |
| GSM506032 |
BAT from per2+/+ mice, biological rep3 |
| GSM506033 |
BAT from per2-/- mice, biological rep1 |
| GSM506034 |
BAT from per2-/- mice, biological rep2 |
| GSM506035 |
BAT from per2-/- mice, biological rep3 |
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| Relations |
| BioProject |
PRJNA125627 |
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