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| Status |
Public on Apr 28, 2022 |
| Title |
Transcriptomic analysis of hepatocellular carcinoma cell lines HepG2 treated with Sorafenib and transfected with miR-200c-3p inhibitor, and miR-222-5p and miR-512-3p mimics |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Hepatocellular carcinoma (HCC) is one of the most common causes of death worldwide and the fourth most prevalent type of cancer. Whereas curative treatments such as liver transplantation, ablation or surgery are optimal for early stages, only paliative treatments are given to intermediate and advanced stages of the disease. Despite the introduction of immune regulators as first-line treatments for advanced stages, Sorafenib is still the standard of care in the clinical practice. In cell lysates, anti-tumoral properties of Sorafenib were related to upregulation of miR-200c-3p (anti-tumoral miRNA) at 6 hours of treatment and downregulation of miR-222-5p and miR-512-3p (pro-tumoral miRNAs) at 24 hours. We have identified these miRNA biomarkers of Sorafenib treatment response in plasma of patients with advanced HCC treated with Sorafenib. In particular, miR-200c-3p has been related to increased survival benefit whereas miR-222-5p and miR-512-3p have been related to worse prognosis. Our study has sequenced HepG2 cells treated with Sorafenib and miR-200c-3p inhibitor, and transfected with miR-222-5p and miR-512-3p mimics to unravel the molecular pathways governing Sorafenib response
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| Overall design |
Transcriptomic analysis of hepatocellular carcinoma cell lines HepG2 treated with Sorafenib and transfected with miR-200c-3p inhibitor, and miR-222-5p and miR-512-3p mimics. Three biological replicates are provided for miRNA and control transfected cells, as well as Sorafenib and control treated cells.
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| Contributor(s) |
de la Cruz-Ojeda P, Muntané J, Schmid T, Brüne B |
| Citation(s) |
36078082 |
| Submission date |
Apr 27, 2022 |
| Last update date |
Sep 15, 2022 |
| Contact name |
Patricia de la Cruz-Ojeda |
| Organization name |
Institute of Biomedicine of Seville
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| Lab |
209
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| Street address |
Avda. Manuel Siurot s/n
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| City |
Sevilla |
| ZIP/Postal code |
41011 |
| Country |
Spain |
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| Platforms (1) |
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| Samples (30)
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| This SubSeries is part of SuperSeries: |
| GSE201697 |
miR-200c-3p, miR-222-5p and miR-512-3p are outcome circulating biomarkers in patients with hepatocarcinoma under Sorafenib treatment |
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| Relations |
| BioProject |
PRJNA832691 |
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