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| Status |
Public on Feb 13, 2010 |
| Title |
Expression data from the mosquito Anopheles gambiae midgut 3h after a protein meal with or without E. coli. |
| Platform organisms |
Plasmodium falciparum; Anopheles gambiae |
| Sample organism |
Anopheles gambiae |
| Experiment type |
Expression profiling by array
|
| Summary |
The Anopheles gambiae midgut harbors bacteria that proliferate upon a blood feed. We used microarrays to examine the midgut gene expression response at early stages (3hours) after an artifitial meal containing heat killed bacteria.
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| Overall design |
Anopheles gambiae G3 mosquitoes 5-6 day-old were fed BSA (20% in PBS with fresh 10 mM sodium bicarbonate) with or without heat killed E. coli (equivalent of 2.5 ml of 0.8 OD) . Three pools of 10 mosquito midguts were dissected after 3h and processed for microarray analysis of gene expression.
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| Contributor(s) |
Molina-Cruz A, Barillas-Mury C |
| Citation(s) |
20223948 |
| |
| Submission date |
Feb 04, 2010 |
| Last update date |
Jan 24, 2013 |
| Contact name |
Alvaro Molina-Cruz |
| E-mail(s) |
amolina-cruz@niaid.nih.gov
|
| Phone |
301-435-2706
|
| Fax |
301-480-0930
|
| Organization name |
NIH
|
| Department |
NIAID
|
| Lab |
LMVR
|
| Street address |
12735 Twinbrook Parkway
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20852 |
| Country |
USA |
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| Platforms (1) |
| GPL1321 |
[Plasmodium_Anopheles] Affymetrix Plasmodium/Anopheles Genome Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA125715 |
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