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| Status |
Public on May 01, 2024 |
| Title |
Maternal Schistosomiasis Bone Marrow B Cell V(D)J scRNAseq |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
We performed V(D)J scRNA sequencing to find transcriptional and B cell receptor repertoire differences in maturing B cells of mice born to mothers chronically infected with Schistosoma mansoni. Cite-seq was used to help identify subpopulations.
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| Overall design |
Bone marrow cells were purified from bone marrow from the femur of mice born to S. mansoni infected and control mothers using a FACs Aria. Sorted cells were DAPI-CD3-CD4-CD8a-CD11b-CD11c-GR-1-Ter119-CD19+B220+IgD+IgM+/-. After sorting, cells were stained with the cell surface protein IgD oligonucleotide-tagged antibody to perform CITE-sequencing. Bone marrow from at least 3 mice were pooled before sorting. Cells were loaded into the 10X Genomics Chromium instrument according to the manufacturer's directions.
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| Contributor(s) |
Gibbs L, Fairfax K, Oviedo JM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 06, 2022 |
| Last update date |
May 01, 2024 |
| Contact name |
Keke Celeste Fairfax |
| E-mail(s) |
keke.fairfax@path.utah.edu
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| Phone |
810-581-5980
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| Organization name |
University of Utah
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| Department |
Pathology
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| Street address |
15 N Medical Drive East
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| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (10)
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| GSM6118821 |
bone marrow, females from infected mothers, rep1, ADT-derived cDNA |
| GSM6118822 |
bone marrow, females from infected mothers, rep1, mRNA-derived cDNA |
| GSM6118823 |
bone marrow, females from infected mothers, rep1, V(D)J-derived cDNA |
| GSM6118824 |
bone marrow, males from infected mothers, rep1, V(D)J-derived cDNA |
| GSM6118825 |
bone marrow, females from uninfected mothers, rep1, ADT-derived cDNA |
| GSM6118826 |
bone marrow, females from uninfected mothers, rep1, mRNA-derived cDNA |
| GSM6118827 |
bone marrow, females from uninfected mothers, rep1, V(D)J-derived cDNA |
| GSM6118828 |
bone marrow, males from uninfected mothers, rep1, ADT-derived cDNA |
| GSM6118829 |
bone marrow, males from uninfected mothers, rep1, mRNA-derived cDNA |
| GSM6118830 |
bone marrow, males from uninfected mothers, rep1, V(D)J-derived cDNA |
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| Relations |
| BioProject |
PRJNA835857 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE202403_RAW.tar |
63.5 Mb |
(http)(custom) |
TAR (of CSV, H5) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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