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GEO help: Mouse over screen elements for information. |
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Status |
Public on Feb 02, 2023 |
Title |
Regulation of Immunological Tolerance by the p53-Inhibitor iASPP [ChIP-seq] |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Maintenance of immunological homeostasis between tolerance and autoimmunity is essential for the prevention of human diseases ranging from autoimmune disease to cancer. Accumulating evidence suggests that p53 can mitigate phagocytosis-induced adjuvanticity thereby promoting immunological tolerance following programmed cell death. Here we identify Inhibitor of Apoptosis Stimulating p53 Protein (iASPP), a negative regulator of p53 transcriptional activity, as a regulator of immunological tolerance. iASPP-deficiency promoted lung adenocarcinoma and pancreatic cancer tumorigenesis, while iASPP-deficient mice were less susceptible to autoimmune disease. Immune responses to iASPP-deficient tumors exhibited hallmarks of immunosuppression, including activated regulatory T cells and exhausted CD8+ T cells. Interestingly, iASPP-deficient tumor cells and tumor-infiltrating myeloid cells, CD4+, and gamma-delta T cells expressed elevated levels of PD-1H, a recently identified transcriptional target of p53 that promotes tolerogenic phagocytosis. Identification of an iASPP/p53 axis of immune homeostasis provides a therapeutic opportunity for both autoimmune disease and cancer.
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Overall design |
ChIP-seq of p53 and total RNA polymerase II in A549 wild-type cells or A549 PPP1R13L knockdown cells.
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Web link |
https://www.nature.com/articles/s41419-023-05567-9
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Contributor(s) |
Akama-Garren EH, Miller P, Carroll TM, Tellier M, Sutendra G, Buti L, Zaborowska J, Goldin RD, Slee E, Szele F, Murphy S, Lu X |
Citation(s) |
36746936 |
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Submission date |
May 07, 2022 |
Last update date |
May 04, 2023 |
Contact name |
Michael Tellier |
E-mail(s) |
mt477@leicester.ac.uk
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Organization name |
University of Leicester
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Department |
Department of Molecular and Cell Biology
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Lab |
Tellier Lab
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Street address |
Lancaster Road
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City |
Leicester |
State/province |
Select State |
ZIP/Postal code |
LE1 7HB |
Country |
United Kingdom |
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Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (32)
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GSM6122600 |
A549, DMSO, p53-CS, R2 |
GSM6122601 |
A549, DMSO, p53-SC |
GSM6122602 |
A549, DMSO, p53, 6h |
GSM6122603 |
A549, DMSO, p53, 8h |
GSM6122604 |
A549, PolII, R2 |
GSM6122605 |
A549, p53-CS, R2 |
GSM6122606 |
A549, p53-SC |
GSM6122607 |
Input, A549, DMSO, 6h |
GSM6122608 |
Input, A549, DMSO, 8h |
GSM6122609 |
Input, A549, DMSO, R2 |
GSM6122610 |
Input, A549, DMSO, p53-SC |
GSM6122611 |
Input, A549, R2 |
GSM6122612 |
Input, A549, p53-SC |
GSM6122613 |
Input, KO, DMSO, 6h |
GSM6122614 |
Input, KO, DMSO, 8h |
GSM6122615 |
Input, KO, DMSO, R2 |
GSM6122616 |
Input, KO, DMSO, p53-SC |
GSM6122617 |
Input, KO, R2 |
GSM6122618 |
Input, KO, p53-SC |
GSM6122619 |
KO, DMSO, PolII, 6h |
GSM6122620 |
KO, DMSO, PolII, 8h |
GSM6122621 |
KO, DMSO, PolII, R2 |
GSM6122622 |
KO, DMSO, p53-CS, R2 |
GSM6122623 |
KO, DMSO, p53-SC |
GSM6122624 |
KO, DMSO, p53, 6h |
GSM6122625 |
KO, DMSO, p53, 8h |
GSM6122626 |
KO, PolII, R2 |
GSM6122627 |
KO, p53-CS, R2 |
GSM6122628 |
KO, p53-SC |
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This SubSeries is part of SuperSeries: |
GSE202445 |
Regulation of Immunological Tolerance by the p53-Inhibitor iASPP |
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Relations |
BioProject |
PRJNA836120 |
Supplementary file |
Size |
Download |
File type/resource |
GSE202443_RAW.tar |
1.8 Gb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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