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Series GSE202443 Query DataSets for GSE202443
Status Public on Feb 02, 2023
Title Regulation of Immunological Tolerance by the p53-Inhibitor iASPP [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Maintenance of immunological homeostasis between tolerance and autoimmunity is essential for the prevention of human diseases ranging from autoimmune disease to cancer. Accumulating evidence suggests that p53 can mitigate phagocytosis-induced adjuvanticity thereby promoting immunological tolerance following programmed cell death. Here we identify Inhibitor of Apoptosis Stimulating p53 Protein (iASPP), a negative regulator of p53 transcriptional activity, as a regulator of immunological tolerance. iASPP-deficiency promoted lung adenocarcinoma and pancreatic cancer tumorigenesis, while iASPP-deficient mice were less susceptible to autoimmune disease. Immune responses to iASPP-deficient tumors exhibited hallmarks of immunosuppression, including activated regulatory T cells and exhausted CD8+ T cells. Interestingly, iASPP-deficient tumor cells and tumor-infiltrating myeloid cells, CD4+, and gamma-delta T cells expressed elevated levels of PD-1H, a recently identified transcriptional target of p53 that promotes tolerogenic phagocytosis. Identification of an iASPP/p53 axis of immune homeostasis provides a therapeutic opportunity for both autoimmune disease and cancer.
 
Overall design ChIP-seq of p53 and total RNA polymerase II in A549 wild-type cells or A549 PPP1R13L knockdown cells.
Web link https://www.nature.com/articles/s41419-023-05567-9
 
Contributor(s) Akama-Garren EH, Miller P, Carroll TM, Tellier M, Sutendra G, Buti L, Zaborowska J, Goldin RD, Slee E, Szele F, Murphy S, Lu X
Citation(s) 36746936
Submission date May 07, 2022
Last update date May 04, 2023
Contact name Michael Tellier
E-mail(s) mt477@leicester.ac.uk
Organization name University of Leicester
Department Department of Molecular and Cell Biology
Lab Tellier Lab
Street address Lancaster Road
City Leicester
State/province Select State
ZIP/Postal code LE1 7HB
Country United Kingdom
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (32)
GSM6122597 A549, DMSO, PolII, 6h
GSM6122598 A549, DMSO, PolII, 8h
GSM6122599 A549, DMSO, PolII, R2
This SubSeries is part of SuperSeries:
GSE202445 Regulation of Immunological Tolerance by the p53-Inhibitor iASPP
Relations
BioProject PRJNA836120

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE202443_RAW.tar 1.8 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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