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| Status |
Public on Mar 01, 2023 |
| Title |
HyperTRIBE uncovers SYNCRIP RNA binding activity in hematopoietic stem cells (HSCs) and multipotent progenitors (MPPs) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
Identifying SYNCRIP RNA-binding targets in HSC and MPP populations, in order to uncover which direct targets influence SYNCRIP's role in maintaining HSCs self renewal capacity.
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| Overall design |
HSCs (Lin-, Sca1+, cKit+, CD150+ CD48-) and MPPs (Lin-, Sca1+, cKit+, all remaining CD150 CD48 cells) from C57BL/6 mice (The Jackson Laboratory, USA) were FACS sorted and transduced with virus expressing either empty vector (MSCV-IRES-GFP) or SYNCRIP-ADAR. 48hrs post transduction cells were sorted for GFP+ expression and used for RNA-seq.
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| Contributor(s) |
Vu LP, Herrejon Chavez F, Chu KL, Kharas MG |
| Citation(s) |
37085479 |
| |
| Submission date |
May 08, 2022 |
| Last update date |
May 12, 2023 |
| Contact name |
Michael Kharas |
| E-mail(s) |
kharasm@mskcc.org
|
| Organization name |
Mamorial Sloan Kettering Cancer Center
|
| Department |
Molecular Pharmacology Program
|
| Lab |
Michael Kharas
|
| Street address |
417 68th street
|
| City |
new york |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA836258 |
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