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Status |
Public on May 12, 2022 |
Title |
Transcriptome-wide impact of season and progesterone in the medio-basal hypothalamus of the goat |
Organism |
Capra hircus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Here we used RNAseq to compare the transcriptomes of the medio-basal hypothalamus of goats killed at two different times of the year (end of January and mid-May) and under different hormonal conditions (follicular and luteal phase, in January only). The current experiment was designed to identify the impact of photoperiod upon the transcriptome and to compare it with that of the ewe. The current design also tested the potential impact of progesterone (P4) upon this transcriptome; ewes were treated with a vaginal fluogestone acetate sponge during the breeding season (January) and killed either 2 days (follicular phase) or 8 days (luteal phase) after estrumate treatment and sponge removal.
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Overall design |
The study was performed in adult Alpine goats. The experiment included 18 goats: 5 for January/Follicular phase; 5 for January/Luteal phase and 8 for mid-May/Anestrus. RNA was extracted from each goat MBH. Then, for each condition two random equimolar mixes of mRNA were used for RNAseq --> 2 biological replicates.
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Contributor(s) |
Dardente H |
Citation missing |
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Submission date |
May 09, 2022 |
Last update date |
May 12, 2022 |
Contact name |
Hugues Dardente |
E-mail(s) |
hugues.dardente@inrae.fr
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Organization name |
INRAE
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Department |
INRAE - Centre Val de Loire
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Lab |
Physiologie de la Reproduction et des Comportements
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Street address |
INRA
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City |
Nouzilly |
ZIP/Postal code |
37380 |
Country |
France |
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Platforms (1) |
GPL19149 |
Illumina HiSeq 2500 (Capra hircus) |
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Samples (6)
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GSM6123299 |
Medio-Basal Hypohtalamus - January/Follicular phase - 1st replicate |
GSM6123300 |
Medio-Basal Hypohtalamus - January/Follicular phase - 2nd replicate |
GSM6123301 |
Medio-Basal Hypohtalamus - January/Luteal phase - 1st replicate |
GSM6123302 |
Medio-Basal Hypohtalamus - January/Luteal phase - 2nd replicate |
GSM6123303 |
Medio-Basal Hypohtalamus - May/Anestrus - 2nd replicate |
GSM6123304 |
Medio-Basal Hypohtalamus - May/Anestrus - 1st replicate |
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Relations |
BioProject |
PRJNA836400 |