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Series GSE202507 Query DataSets for GSE202507
Status Public on May 12, 2022
Title Transcriptome-wide impact of season and progesterone in the medio-basal hypothalamus of the goat
Organism Capra hircus
Experiment type Expression profiling by high throughput sequencing
Summary Here we used RNAseq to compare the transcriptomes of the medio-basal hypothalamus of goats killed at two different times of the year (end of January and mid-May) and under different hormonal conditions (follicular and luteal phase, in January only). The current experiment was designed to identify the impact of photoperiod upon the transcriptome and to compare it with that of the ewe. The current design also tested the potential impact of progesterone (P4) upon this transcriptome; ewes were treated with a vaginal fluogestone acetate sponge during the breeding season (January) and killed either 2 days (follicular phase) or 8 days (luteal phase) after estrumate treatment and sponge removal.
 
Overall design The study was performed in adult Alpine goats. The experiment included 18 goats: 5 for January/Follicular phase; 5 for January/Luteal phase and 8 for mid-May/Anestrus. RNA was extracted from each goat MBH. Then, for each condition two random equimolar mixes of mRNA were used for RNAseq --> 2 biological replicates.
 
Contributor(s) Dardente H
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Submission date May 09, 2022
Last update date May 12, 2022
Contact name Hugues Dardente
E-mail(s) hugues.dardente@inrae.fr
Organization name INRAE
Department INRAE - Centre Val de Loire
Lab Physiologie de la Reproduction et des Comportements
Street address INRA
City Nouzilly
ZIP/Postal code 37380
Country France
 
Platforms (1)
GPL19149 Illumina HiSeq 2500 (Capra hircus)
Samples (6)
GSM6123299 Medio-Basal Hypohtalamus - January/Follicular phase - 1st replicate
GSM6123300 Medio-Basal Hypohtalamus - January/Follicular phase - 2nd replicate
GSM6123301 Medio-Basal Hypohtalamus - January/Luteal phase - 1st replicate
Relations
BioProject PRJNA836400

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Supplementary file Size Download File type/resource
GSE202507_Dardente_Foll_vs_Anes_Raw_Counts_RNA-Seq.txt.gz 349.7 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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