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| Status |
Public on Mar 22, 2023 |
| Title |
Isoginkgetin induces autophagic cell death in hepatocellular carcinoma [ChIP-seq 2] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Purpose: Our previous studies have identified isoginkgetin as an inducer of autophagic cell death in hepatocellular carcinoma. We set out to analyze how isoginkgetin regulates the genome-wide enhancer activity in HepG2 cells.
Methods: ChIP-seqs for H3K4me, H4K8ac, H3K27me3 and H3K9ac were performed in HepG2 followed by treatment with 20 μM isoginkgetin for 24 h.
Conclusions: Isoginkgetin treatment reduces the expression of glucose transporters and inhibts SLC2A1 enhancer activity in HepG2 cells.
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| Overall design |
ChIP-seq was performed in HepG2 cells followed by treatment with 20 μM Isoginkgetin for 24 h. The genome-wide enhancer activity was studied by H3K4me, H4K8ac, H3K27me3 and H3K9ac ChIP sequencing.
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| |
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| Contributor(s) |
Yao J, Gao C, Zeng X |
| Citation(s) |
36048765 |
| |
| Submission date |
May 10, 2022 |
| Last update date |
Mar 23, 2023 |
| Contact name |
Jie Yao |
| E-mail(s) |
yaojie@whu.edu.cn
|
| Phone |
18771075190
|
| Organization name |
wuhan university
|
| Street address |
wuhan hubei province
|
| City |
wuhan |
| State/province |
湖北 |
| ZIP/Postal code |
430072 |
| Country |
China |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE188535 |
Isoginkgetin induces autophagic cell death in hepatocellular carcinoma |
|
| Relations |
| BioProject |
PRJNA836776 |
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