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| Status |
Public on May 30, 2023 |
| Title |
Schwann Cells Shape Tumor Cells and Cancer-Associated Fibroblasts in the Pancreatic Ductal Adenocarcinoma Microenvironment [ST] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Neuropathy is a feature more frequently observed in pancreatic ductal adenocarcinoma (PDAC) than other tumors. Schwann cells, the most prevalent cell in peripheral nerves, migrate toward tumor cells and associate with poor prognosis in PDAC. To unveil the effects of Schwann cells on the neuro-stroma niche, single-cell RNA-sequencing and microarray-based spatial transcriptome analysis of PDAC tissues were performed. Results suggested that Schwann cells may drive tumor cells and cancer-associated fibroblasts (CAFs) to more malignant subtypes: basal-like and inflammatory CAFs (iCAFs), respectively. Moreover, in vitro and in vivo assays demonstrated that Schwann cells enhanced the proliferation and migration of PDAC cells via Midkine and promoted the switch of CAFs to iCAFs via interleukin-1a. By Schwann cells condition medium, tumor cells together with iCAFs accelerate PDAC progression. Thus, we revealed, for the first time, that Schwann cells induce malignant subtypes of tumor cells and CAFs in the PDAC milieu.
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| Overall design |
spatial transcriptome was performed in 4 PDAC patient tumor samples with neuropathy.
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| Contributor(s) |
Xue M, Zhu Y, Jiang Y, Han L, Shi M, Su R, Wang L, Xiong C, Wang C, Wang T, Wu D, Cao Y, Dong L, Bai F, Zhao S, Deng X, Peng C, Li H, Chen J, Shen B, Jiang L, Chen H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 11, 2022 |
| Last update date |
May 31, 2023 |
| Contact name |
Meilin Xue |
| E-mail(s) |
xuemeilin@sjtu.edu.cn
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| Organization name |
Ruijin Hospital, Shanghai Jiao Tong University School of Medicine
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| Street address |
197 Ruijin 2nd Road
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| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200020 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA837200 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE202740_RAW.tar |
1017.0 Mb |
(http)(custom) |
TAR (of MTX, TIFF, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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