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| Status |
Public on Mar 11, 2011 |
| Title |
The effect of TuMV on translation initiation in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
Arabidopsis ecotype Col-0 expressing RPL18 fused to His & Flag epitopes (RPL18-HF) under 35S control was used to discern the effects of Turnip mosaic virus (TuMV) infection on host translation initiation. Plants were grown in LC-1 soil in 12 hour days and infected by TuMV-GFP or mock-inoculated just prior to sending up bolts. Samples were taken from rosette leaves 10 days after inoculation. Only tissue fluorescing GFP was selected from the virus-infected samples. Similar tissue was sampled from mock-infected leaves. FLAG antibody was used to isolate RPL18-HF. The RNA co-immunoprecipitated with RPL18-HF is fully translation-initiated. This translation-initiated RNA, also referred to as polysomal RNA, was isolated and compared to total RNA under both mock and TuMV-infected states to find TuMV-induced changes in host translation initiation. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Jackson Moeller. The equivalent experiment is AT42 at PLEXdb.]
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| Overall design |
pathogen infection: TuMV inoculated - RNA fraction: polysomal RNA(3-replications); pathogen infection: TuMV inoculated - RNA fraction: total RNA(3-replications); pathogen infection: mock inoculated - RNA fraction: polysomal RNA(3-replications); pathogen infection: mock inoculated - RNA fraction: total RNA(3-replications)
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| Web link |
http://www.plexdb.org/modules/PD_browse/experiment_browser.php?experiment=AT42
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| |
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| Contributor(s) |
Moeller J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 11, 2010 |
| Last update date |
Aug 15, 2018 |
| Contact name |
PLEXdb Curator, Sudhansu Dash |
| Organization name |
Iowa State University
|
| Department |
Virtual Reality Application Center
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| Lab |
PLEXdb
|
| Street address |
1023 Crop Genomes Informatics Lab (Iowa State University)
|
| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (12)
|
| GSM508436 |
pathogen infection: TuMV inoculated - RNA fraction: polysomal RNA - rep1 |
| GSM508437 |
pathogen infection: TuMV inoculated - RNA fraction: polysomal RNA - rep2 |
| GSM508438 |
pathogen infection: TuMV inoculated - RNA fraction: polysomal RNA - rep3 |
| GSM508439 |
pathogen infection: TuMV inoculated - RNA fraction: total RNA - rep1 |
| GSM508440 |
pathogen infection: TuMV inoculated - RNA fraction: total RNA - rep2 |
| GSM508441 |
pathogen infection: TuMV inoculated - RNA fraction: total RNA - rep3 |
| GSM508442 |
pathogen infection: mock inoculated - RNA fraction: polysomal RNA - rep1 |
| GSM508443 |
pathogen infection: mock inoculated - RNA fraction: polysomal RNA - rep2 |
| GSM508444 |
pathogen infection: mock inoculated - RNA fraction: polysomal RNA - rep3 |
| GSM508445 |
pathogen infection: mock inoculated - RNA fraction: total RNA - rep1 |
| GSM508446 |
pathogen infection: mock inoculated - RNA fraction: total RNA - rep2 |
| GSM508447 |
pathogen infection: mock inoculated - RNA fraction: total RNA - rep3 |
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA125369 |
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