|
| Status |
Public on Jul 01, 2022 |
| Title |
In vitro generation of brain regulatory T cells by co-culturing with astrocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We developed a culture method that confers brain Treg characteristics in vitro. Naive Tregs from the spleen were activated and efficiently amplified by T-cell receptor (TCR) stimulation in the presence of primary astrocytes.
|
| |
|
| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for Treg cells from spleen (SP-Treg) and brain with ischemic stroke (Isckemia-Treg) and in vitro culture (iB-Treg(A), iB-Treg(B), iB-Treg(C)).
|
| |
|
| Contributor(s) |
Yamamoto S, Matsui A, Ohyagi M, Kikutake C, Harada Y, Iizuka-Koga M, Suyama M, Yoshimura A, Ito M |
| Citation(s) |
35911740 |
| |
| Submission date |
May 15, 2022 |
| Last update date |
Aug 03, 2022 |
| Contact name |
Minako Ito |
| E-mail(s) |
minakoito@bioreg.kyushu-u.ac.jp
|
| Organization name |
Kyushu University
|
| Street address |
3-1-1 Maidashi, Higashi-ku
|
| City |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA838355 |
Less...
More...