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Series GSE203127 Query DataSets for GSE203127
Status Public on Jan 31, 2024
Title Xrp1 governs the stress response program to spliceosome dysfunction
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary We investigated the changes in gene expression in response to reduced levels of Ecdysoneless (Ecd) in the developing wing disc of Drosophila. To do this we utilized the GAL4/UAS system to express UAS-ecd-RNAi in the nubbin-domain (nub-Gal4, UAS-mRFP, UAS-ecd-RNAi) of the wing disc and used Nub-Gal4/+ wing discs as controls.  We quantified transcriptomic expression analysis from four biological replicates for both experimental groups
 
Overall design mRNA profiles were generated by single-end, total RNA Illumina sequencing (HiSeq2500) from wandering L3 stage wing discs of nub-Gal4, UAS-mRFP, UAS-ecd-RNAi and nub-Gal4, UAS-mRFP Drosophila larvae.
Web link https://doi.org/10.1093/nar/gkae055
 
Contributor(s) Stankovic D, Tain L, Uhlirova M
Citation(s) 38303573
Submission date May 16, 2022
Last update date Apr 02, 2024
Contact name Mirka Uhlirova
E-mail(s) mirka.uhlirova@uni-koeln.de
Organization name CECAD Research Center Cologne
Department Institute for Genetics
Lab Uhlirova Lab
Street address Joseph-Stelzmann Str. 26
City Cologne
State/province NRW
ZIP/Postal code 50931
Country Germany
 
Platforms (1)
GPL13304 Illumina HiSeq 2000 (Drosophila melanogaster)
Samples (8)
GSM6157206 nub>mRFP replicate 1
GSM6157207 nub>mRFP replicate 2
GSM6157208 nub>mRFP replicate 3
Relations
BioProject PRJNA838615

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Supplementary file Size Download File type/resource
GSE203127_Supplementary_Dataset_1_DESeq2_ecd-RNAi_regulated_genes.csv.gz 597.0 Kb (ftp)(http) CSV
GSE203127_Supplementary_Dataset_2_DESeq2_normalized_counts.txt.gz 709.9 Kb (ftp)(http) TXT
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