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Status |
Public on Jan 31, 2024 |
Title |
Xrp1 governs the stress response program to spliceosome dysfunction |
Organism |
Drosophila melanogaster |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We investigated the changes in gene expression in response to reduced levels of Ecdysoneless (Ecd) in the developing wing disc of Drosophila. To do this we utilized the GAL4/UAS system to express UAS-ecd-RNAi in the nubbin-domain (nub-Gal4, UAS-mRFP, UAS-ecd-RNAi) of the wing disc and used Nub-Gal4/+ wing discs as controls. We quantified transcriptomic expression analysis from four biological replicates for both experimental groups
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Overall design |
mRNA profiles were generated by single-end, total RNA Illumina sequencing (HiSeq2500) from wandering L3 stage wing discs of nub-Gal4, UAS-mRFP, UAS-ecd-RNAi and nub-Gal4, UAS-mRFP Drosophila larvae.
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Web link |
https://doi.org/10.1093/nar/gkae055
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Contributor(s) |
Stankovic D, Tain L, Uhlirova M |
Citation(s) |
38303573 |
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Submission date |
May 16, 2022 |
Last update date |
Apr 02, 2024 |
Contact name |
Mirka Uhlirova |
E-mail(s) |
mirka.uhlirova@uni-koeln.de
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Organization name |
CECAD Research Center Cologne
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Department |
Institute for Genetics
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Lab |
Uhlirova Lab
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Street address |
Joseph-Stelzmann Str. 26
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City |
Cologne |
State/province |
NRW |
ZIP/Postal code |
50931 |
Country |
Germany |
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Platforms (1) |
GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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Samples (8)
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Relations |
BioProject |
PRJNA838615 |