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| Status |
Public on Mar 10, 2023 |
| Title |
Identifying endogenous superior and inferior neural progenitor cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Stem cell competition controls organ size, maintains tissue homeostasis and optimizes long-term tissue function through eliminating cells less fit than their neighbors, but whether competitive interaction occurs between neural stem cells in the developing brain and its mechanistic drivers remains enigmatic. We applied single-cell RNA sequencing of mouse E11.5 medial cortex and bulk RNA sequencing of AnnexinV positive, PI negative cells to identify the loser cell in the natural state.
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| Overall design |
Single cell sequencing of mouse E11.5 medial cortex. Bulk RNA sequencing of AnnexinV positive, PI negative and AnnexinV, PI double negative cells.
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| |
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| Contributor(s) |
Chen Z, Sun X, Wu Q |
| Citation(s) |
37054704 |
| Submission date |
May 17, 2022 |
| Last update date |
Jun 09, 2023 |
| Contact name |
Zhenhua Chen |
| E-mail(s) |
czh@genetics.ac.cn
|
| Organization name |
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
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| Department |
State Key Laboratory of Molecular Development Biology
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| Lab |
Wu
|
| Street address |
West Beichen Road, Olympic Village, Beijing
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| City |
Beijing |
| State/province |
China |
| ZIP/Postal code |
100000 |
| Country |
China |
| |
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| Platforms (2) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL28457 |
DNBSEQ-G400 (Mus musculus) |
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| Samples (15)
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| GSM6160907 |
Annexin and PI double negative sample rep1 |
| GSM6160908 |
Annexin and PI double negative sample rep2 |
| GSM6160909 |
Annexin and PI double negative sample rep3 |
| GSM6160910 |
Annexin positive and PI negative sample rep1 |
| GSM6160911 |
Annexin positive and PI negative sample rep2 |
| GSM6160912 |
Annexin positive and PI negative sample rep3 |
| GSM6160913 |
Annexin and PI double negative sample rep4 |
| GSM6160914 |
Annexin and PI double negative sample rep5 |
| GSM6160915 |
Annexin and PI double negative sample rep6 |
| GSM6160916 |
Annexin positive and PI negative sample rep4 |
| GSM6160917 |
Annexin positive and PI negative sample rep5 |
| GSM6160918 |
Annexin positive and PI negative sample rep6 |
| GSM6160919 |
R366 E11.5 Medial cortex |
| GSM6160920 |
R367 E11.5 Medial cortex |
| GSM6160921 |
R368 E11.5 Medial cortex |
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| Relations |
| BioProject |
PRJNA838936 |
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