|
| Status |
Public on Feb 15, 2010 |
| Title |
Hairpin RNA induces secondary small interfering RNA synthesis and silencing in trans in fission yeast |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We provide evidence of the generation of Rdp1-mediated secondary siRNAs in vivo in fission yeast. Secondly, we show that the presence of Ago1-associated siRNAs does not guarantee robust silencing.
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| Overall design |
We obtained sequences from gel purified small RNAs of wt and rdp1delta fission yeast cells and FLAG-ago1 associated small RNAs.
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| Contributor(s) |
Simmer F, Buscaino A, Kos-Braun IC, Kagansky A, Boukaba A, Urano T, Kerr AR, Allshire RC |
| Citation(s) |
20062003 |
| |
| Submission date |
Feb 15, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Alastair Robert Kerr |
| E-mail(s) |
alastair.kerr@ed.ac.uk
|
| Organization name |
University of Edinburgh
|
| Department |
Wellcome Centre for Cell Biology
|
| Lab |
Bioinformatics Core Facility
|
| Street address |
2.21 Michael Swann Building, Kings Buildings, Mayfield Road
|
| City |
Edinburgh |
| State/province |
Midlothian |
| ZIP/Postal code |
EH9 3JR |
| Country |
United Kingdom |
| |
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| Platforms (2) |
| GPL9453 |
Illumina Genome Analyzer II (Schizosaccharomyces pombe) |
| GPL9854 |
Illumina Genome Analyzer (Schizosaccharomyces pombe) |
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| Samples (3) |
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| Relations |
| SRA |
SRP001896 |
| BioProject |
PRJNA125397 |
