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| Status |
Public on Jan 12, 2023 |
| Title |
Geometric engineering of organoid culture for enhanced organogenesis in a dish |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Here we introduce a facile, scalable engineering approach to enable long-term development and maturation of organoids. We have redesigned the configuration of conventional organoid culture to develop a platform that converts single injections of stem cell suspensions to radial arrays of organoids that can be maintained for extended periods without the need for passaging. Using this system, we demonstrate accelerated production of intestinal organoids with significantly enhanced structural and functional maturity, and their continuous development for over 4 weeks. Furthermore, we present a patient-derived organoid model of inflammatory bowel disease (IBD) and its interrogation using single-cell RNA sequencing to demonstrate its ability to reproduce key pathological features of IBD. Finally, we describe the extension of our approach to engineer vascularized, perfusable human enteroids, which can be used to model innate immune responses in IBD. This work provides an immediately deployable platform technology towards engineering more realistic organ-like structures in a dish.
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| Overall design |
Intestinal epithelial cells isolated from enteroids of the control and IBD patients were analyzed using scRNAseq.
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| Contributor(s) |
Huh DD, Park SE |
| Citation(s) |
36280722 |
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| Submission date |
May 19, 2022 |
| Last update date |
Jan 12, 2023 |
| Contact name |
Dan Dongeun Huh |
| Organization name |
University of Pennsylvania
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| Street address |
210 South 33rd Street 315S Skirkanich Hall
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| City |
Philadelphia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (5)
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| GSM6170835 |
IBD-OCTOPUS-d14, replicate 1, scRNAseq |
| GSM6170836 |
Normal-OCTOPUS-d7, replicate 1, scRNAseq |
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| Relations |
| BioProject |
PRJNA839719 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE203380_RAW.tar |
922.8 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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