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| Status |
Public on May 01, 2024 |
| Title |
Maternal and Chronic Schistosoma Infection Bone Marrow B cell scRNAseq |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
We performed scRNA sequencing to find transcriptional differences in early B cell populations in the bone marrow of mice born to mothers chronically infected with Schistosoma mansoni compared to their mothers. Cite-seq was used to help identify cluster types using surface protein markers.
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| Overall design |
Hematopoietic stem and progenitor cells and B cells were purified from bone marrow from the femur of mice born to S. mansoni infected and control mothers using a FACs Aria. Sorted cells were DAPI-CD3-CD4-CD8a-CD11b-CD11c-GR-1-Ter119-CD19-. CD19 was used in place of B220 to enrich for pre-pro B cells, but the antibody was ineffective, allowing for all B cell types to be sorted. After sorting, cells were stained with the cell surface proteins CD127, CD124, CD117, Sca-1, CD16/32 and CD138 with oligonucleotide-tagged antibodies to perform CITE-sequencing. Bone marrow from at least 3 mice were pooled before sorting. Cells were loaded into the BD Rhapsody instrument according to the manufacturer's directions.
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| Contributor(s) |
Gibbs L, Fairfax K, Oviedo JM |
| Citation missing |
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| Submission date |
May 19, 2022 |
| Last update date |
May 01, 2024 |
| Contact name |
Keke Celeste Fairfax |
| E-mail(s) |
keke.fairfax@path.utah.edu
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| Phone |
810-581-5980
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| Organization name |
University of Utah
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| Department |
Pathology
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| Street address |
15 N Medical Drive East
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| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| GSM6170874 |
bone marrow, Schistosoma mansoni chronically infected females, rep1, ADT and mRNA-derived combined cDNA |
| GSM6170875 |
bone marrow, non-infected (control) females, rep1, ADT and mRNA-derived combined cDNA |
| GSM6170876 |
bone marrow, males born to control females, rep1, ADT and mRNA-derived combined cDNA |
| GSM6170877 |
bone marrow, males born to Schistosoma mansoni chronically infected females, rep1, ADT and mRNA-derived combined cDNA |
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| Relations |
| BioProject |
PRJNA839726 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE203389_RAW.tar |
630.0 Mb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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