NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE203537 Query DataSets for GSE203537
Status Public on Mar 13, 2023
Title Next Generation Sequencing Facilitates analysis of IL17 EGFP positive and negative cells from small intestine (SI) lamina propia (lp) genes expression
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We performed the bulk RNA seq on IL17 EGFP positive and negative cells from small intestine (SI) lamina propia (lp) of antibiotic treated XBP1ΔIEC mice and found the different genes expression in the setting of IEC ER stress induced Th17 positive and negative cells
 
Overall design Two hundred Il17a-egfp positive (CD45+TCRβ+CD4+ Il17a-egfp+) or negative T cells (CD45+TCRβ+CD4+ Il17a-egfp-) of antibiotic treated Xbp1Two hundred Il17a-egfp positive (CD45+TCRβ+CD4+ Il17a-egfp+) or negative T cells (CD45+TCRβ+CD4+ Il17a-egfp-) of antibiotic treated Xbp1ΔIEC mice were sorted by flow cytometry and used as input material. The RNA extraction and cDNA was generated following the smart-seq2 protocol as previous described(Picelli, Faridani et al; Nat Protoc ;2014). The library preparation and sequencing were performed at the Biopolymers Facility at the Harvard Medical School on Illumina NextSeq 500 sequencer to generate 75-bp paired-end reads.
Web link https://www.cell.com/immunity/fulltext/S1074-7613(23)00092-4
 
Contributor(s) Duan J
Citation(s) 36917985
Submission date May 22, 2022
Last update date Jun 12, 2023
Contact name jinzhi duan
E-mail(s) jduan2@bwh.harvard.edu
Phone 6175257369
Organization name Brigham and Women' hospital
Street address 6th Floor, Room 641, NRB, 77 Louis Pasteur Avenue
City Boston
State/province Massachusetts
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM6175995 il17-egfp negative l1
GSM6175996 il17-egfp negative l2
GSM6175997 il17-egfp negative l3
Relations
BioProject PRJNA841347

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE203537_IL17_EGFP_POSITIVE_AND_NEGATIVE.txt.gz 797.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.