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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 01, 2010 |
| Title |
Regulation of chondrogenesis in early murine limb mesenchyme by BMP signals |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Numerous studies have established a critical role for BMP signaling in skeletal development. In the developing axial skeleton, sequential SHH and BMP signals are required for specification of a chondrogenic fate in somitic tissue. A similar paradigm is thought to operate in the limb, but the signals involved are unclear. To investigate the nature of these signals we examined BMP action in mesenchymal populations derived from the early murine limb bud (~ E10.5). These populations exhibited a graded response to BMPs, in which early limb mesenchymal (EL) cells (from the distal hind limb) displayed an anti-chondrogenic response, whereas BMPs promoted chondrogenesis in older cell populations. To better understand the molecular basis of disparate BMP action in these various populations, gene expression profiling with Affymetrix microarrays was employed to identify BMP-regulated genes. These analyses showed that BMPs induced a distinct gene expression pattern in the EL cultures versus later mesenchymal limb populations (IM and LT).
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| Overall design |
Mouse embryos at gestational age E10.5 were collected and various portions of the limb were micro-dissected. These led to the generation of three populations of cells, early (EL) limb mesenchymal cells from the distal half of the hind limb, an intermediate (IM) population derived from the distal 1/3 of the fore limb, and a later (LT) population from the proximal 2/3 of the fore limb. Mesenchymal cells were isolated and cultured with and without BMP4 treatment. RNA was extracted from cultures at either Day 0,1 or 2, labelled and hybridized to Affymetrix 430 2.0 microarrays. For each time point, RNA was collected from two biological replicates for each treatment condition.
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| Contributor(s) |
Underhill TM |
| Citation(s) |
20501701 |
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| Submission date |
Feb 17, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Tully Michael Underhill |
| E-mail(s) |
tunderhi@brc.ubc.ca
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| Phone |
604-822-5833
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| Organization name |
University of British Columbia
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| Department |
Biomedical Research Centre
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| Street address |
2222 Health Sciences Mall
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| City |
Vancouver |
| State/province |
British Columbia |
| ZIP/Postal code |
V6T 1Z3 |
| Country |
Canada |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (30)
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| GSM511003 |
intermediate at day 0, control, biological rep1 |
| GSM511004 |
intermediate at day 0, control, biological rep2 |
| GSM511005 |
late at day 0, control, biological rep1 |
| GSM511006 |
late at day 0, control, biological rep2 |
| GSM511007 |
early at day 0, control, biological rep1 |
| GSM511008 |
early at day 0, control, biological rep2 |
| GSM511009 |
intermediate at day 1, control, biological rep1 |
| GSM511010 |
intermediate at day 1, control, biological rep2 |
| GSM511011 |
intermediate at day 1, BMP4, biological rep1 |
| GSM511012 |
intermediate at day 1, BMP4, biological rep2 |
| GSM511013 |
late at day 1, control, biological rep1 |
| GSM511014 |
late at day 1, control, biological rep2 |
| GSM511015 |
late at day 1, BMP4, biological rep1 |
| GSM511016 |
late at day 1, BMP4, biological rep2 |
| GSM511017 |
early at day 1, control, biological rep1 |
| GSM511018 |
early at day 1, control, biological rep2 |
| GSM511019 |
early at day 1, BMP4, biological rep1 |
| GSM511020 |
early at day 1, BMP4, biological rep2 |
| GSM511021 |
intermediate at day 2, control, biological rep1 |
| GSM511022 |
intermediate at day 2, control, biological rep2 |
| GSM511023 |
intermediate at day 2, BMP4, biological rep1 |
| GSM511024 |
intermediate at day 2, BMP4, biological rep2 |
| GSM511025 |
late at day 2, control, biological rep1 |
| GSM511026 |
late at day 2, control, biological rep2 |
| GSM511027 |
late at day 2, BMP4, biological rep1 |
| GSM511028 |
late at day 2, BMP4, biological rep2 |
| GSM511029 |
early at day 2, control, biological rep1 |
| GSM511030 |
early at day 2, control, biological rep2 |
| GSM511031 |
early at day 2, BMP4, biological rep1 |
| GSM511032 |
early at day 2, BMP4, biological rep2 |
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| Relations |
| BioProject |
PRJNA125529 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20398_RAW.tar |
134.9 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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