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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 01, 2011 |
| Title |
The effects of aging vs. alpha7 nAChR subunit deficiency on the mouse brain transcriptome |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Aging is accompanied by expression changes in multiple genes and the brain is one of the tissues most vulnerable to aging. Since the alpha7 nicotinic acetylcholine receptor (nAChR) subunit has been associated with neurodevelopmental disorders and cognitive decline during aging, we hypothesized that its absence might affect gene expression profiles in aged brains. To study whether transcriptional changes occur due to aging, alpha7 deficiency or both, we analyzed whole brain transcriptomes of young (8 week) and aged (2 year) alpha7 deficient and wild-type control mice, using Mouse Genome 430 2.0 microarray. Highly significant expression changes were detected in 47 and 1543 genes (after Bonferroni and FDR correction) in the brains of aged mice compared to young mice, regardless of their genotype. These included genes involved in immune system function and ribosome structure, as well as genes that were previously demonstrated as differentially expressed in aging human brains. Genotype-dependent changes were detected in only 3 genes, Chrna7 which encodes the alpha7 nAChR subunit, and two closely linked genes, likely due to a mouse background effect. Expression changes dependent on age-genotype interaction were detected in 207 genes (with a low significance threshold). Age-dependent differential expression levels were approved in all nine genes that were chosen for validation by real-time RT-PCR. Our results suggest that the robust effect of aging on brain transcription clearly overcomes the almost negligible effect of alpha7 nAChR subunit deletion, and that germline deficiency of this subunit has a minor effect on brain expression profile in aged mice.
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| Overall design |
Total of 20 samples. 10 from young adult mice (8 weeks) and 10 from aged mice (2 years). In each age group, 5 were wild-type control mice and 5 alpha7 nAChR subunit defecient mice
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| Contributor(s) |
Kedmi M, Orr-Urtreger A |
| Citation(s) |
20526689 |
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| Submission date |
Feb 18, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Merav Kedmi |
| E-mail(s) |
meravic@hotmail.com
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| Organization name |
Tel Aviv Sourasky Medical Center
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| Street address |
6 Weizmann street
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| City |
Tel Aviv |
| ZIP/Postal code |
64239 |
| Country |
Israel |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (20)
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| GSM511619 |
Wild-Type, Young_a7_1072 |
| GSM511620 |
Wild-Type, Young_a7_556 |
| GSM511621 |
Wild-Type, Aged_a7_708 |
| GSM511622 |
Wild-Type, Aged_a7_744 |
| GSM511623 |
Wild-Type, Aged_a7_758 |
| GSM511624 |
Wild-Type, Aged_a7_759 |
| GSM511625 |
Wild-Type, Aged_a7_765 |
| GSM511626 |
alpha7-/-, Young_a7_1062 |
| GSM511627 |
alpha7-/-, Young_a7_1066 |
| GSM511628 |
alpha7-/-, Young_a7_1067 |
| GSM511629 |
alpha7-/-, Young_a7_1069 |
| GSM511630 |
alpha7-/-, Young_a7_1073 |
| GSM511631 |
alpha7-/-, Aged_a7_52 |
| GSM511632 |
alpha7-/-, Aged_a7_53 |
| GSM511633 |
alpha7-/-, Aged_a7_751 |
| GSM511634 |
alpha7-/-, Aged_a7_752 |
| GSM511635 |
alpha7-/-, Aged_a7_764 |
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| Relations |
| BioProject |
PRJNA125551 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20411_RAW.tar |
353.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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