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Series GSE20431 Query DataSets for GSE20431
Status Public on Jan 24, 2011
Title Gene expression profiling on FePro labeled bone marrow stromal cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Superparamagnetic iron oxide nanoparticles (SPION) are increasingly used to label human bone marrow stromal cells (BMSCs, also called mesenchymal stem cells) to monitor their fate by in vivo MRI and histology after Prussian blue (PB) staining. SPION-labeling appears to be safe as assessed by in vitro differentiation of BMSCs, however, we chose to determine the effect of labeling on maintaining the “stemness” of cells within the BMSC population. Colony forming efficiency, CD146 expression, gene expression profiling, bone and myelosupportive stroma formation in vivo were evaluated. SPION-labeling did not alter these assays. Equivalent bone with host hematopoiesis was formed by SPION-labeled and unlabeled BMSCs. PB+ adipocytes were noted, definitively demonstrating their donor origin, as well as PB+ pericytes, indicative of self-renewal of the stem cell in the BMSC population. This study confirms that SPION labeling does not alter the differentiation potential of the subset of stem cells within BMSCs.
 
Overall design We performed gene expression profiling on BMSCs labeled with FePro. For comparison, gene express profiling on human embryonic stem cells (hES) WA09 (H9) and adult cells: fibroblasts (FB), endothelial cells (EC) and smooth muscle cells (SMC) was conducted. Total RNA from PBMCs pooled from six normal donors was amplified into aRNA to serve as the reference.
 
Citation(s) 20628641
Submission date Feb 19, 2010
Last update date Jan 18, 2013
Contact name Francesco Maria Marincola
E-mail(s) fmarincola@sidra.org
Phone 301-793-8210
Organization name Sidra Medical and Research Center
Street address Al Nasr Tower, AL Corniche Street, PO Box 26999
City Doha
ZIP/Postal code PO Box 26999
Country Qatar
 
Platforms (2)
GPL7088 CCDTM Hs_CCDTM36k - version 1
GPL10077 CCDTM Hs_CCDTM36k - version 1 (derived from GPL7088)
Samples (15)
GSM307451 LFB - mAdbID:94275
GSM307454 WA09 (H9) - replicate 1 - mAdbID:94280
GSM307458 UASMC - mAdbID:94287
Relations
BioProject PRJNA125599

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20431_RAW.tar 54.9 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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