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Series GSE20439 Query DataSets for GSE20439
Status Public on Feb 20, 2010
Title Expression data from cumulus cells from C57Bl/6 and Ptger2 (EP2) KO mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary To understand the role of prostaglandin (PG) receptor EP2 (Ptger2) signaling in ovulation and fertilization, we investigated time-dependent expression profiles in wild-type (WT) and Ptger2-/- cumuli before and after ovulation by using microarrays.
 
Overall design We used Affymetrix U74A ver2 microarrays to investigate gonadotropin-induced gene expression profiles in WT and EP2KO cumulus cells before and after ovulation. Immature mice were primed with pregnant mare serum gonadotropin (PMSG) as an FSH-like stimulation. The cumulus-oocyte complexes (COCs) were isolated from mice 48 h after PMSG injection (H0) and from PMSG-primed mice exposed to human chorionic gonadotropin (hCG) as an LH-like stimulation for 3, 9 or 14 h (H3, H9 or H14, respectively).
 
Contributor(s) Tamba S, Yodoi R, Morimoto K, Inazumi T, Okuno Y, Segi-Nishida E, Sugimoto Y
Citation(s) 20399827
Submission date Feb 19, 2010
Last update date Feb 18, 2018
Contact name Eri Segi-Nishida
E-mail(s) eri.segi.nishida@rs.tus.ac.jp
Organization name Tokyo University of Science
Street address Niijuku 6-3-1
City Tokyo
State/province Tokyo
ZIP/Postal code 125-8585
Country Japan
 
Platforms (1)
GPL81 [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array
Samples (8)
GSM512589 WT_Cumulus_H0
GSM512590 WT_Cumulus_H3
GSM512591 WT_Cumulus_H9
Relations
BioProject PRJNA125493

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20439_RAW.tar 23.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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