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Status |
Public on Feb 20, 2010 |
Title |
Expression data from cumulus cells from C57Bl/6 and Ptger2 (EP2) KO mice |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
To understand the role of prostaglandin (PG) receptor EP2 (Ptger2) signaling in ovulation and fertilization, we investigated time-dependent expression profiles in wild-type (WT) and Ptger2-/- cumuli before and after ovulation by using microarrays.
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Overall design |
We used Affymetrix U74A ver2 microarrays to investigate gonadotropin-induced gene expression profiles in WT and EP2KO cumulus cells before and after ovulation. Immature mice were primed with pregnant mare serum gonadotropin (PMSG) as an FSH-like stimulation. The cumulus-oocyte complexes (COCs) were isolated from mice 48 h after PMSG injection (H0) and from PMSG-primed mice exposed to human chorionic gonadotropin (hCG) as an LH-like stimulation for 3, 9 or 14 h (H3, H9 or H14, respectively).
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Contributor(s) |
Tamba S, Yodoi R, Morimoto K, Inazumi T, Okuno Y, Segi-Nishida E, Sugimoto Y |
Citation(s) |
20399827 |
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Submission date |
Feb 19, 2010 |
Last update date |
Feb 18, 2018 |
Contact name |
Eri Segi-Nishida |
E-mail(s) |
eri.segi.nishida@rs.tus.ac.jp
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Organization name |
Tokyo University of Science
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Street address |
Niijuku 6-3-1
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City |
Tokyo |
State/province |
Tokyo |
ZIP/Postal code |
125-8585 |
Country |
Japan |
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Platforms (1) |
GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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Samples (8)
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Relations |
BioProject |
PRJNA125493 |