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| Status |
Public on Mar 01, 2010 |
| Title |
CXCL4 induces a unique transcriptome in monocyte-derived macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Human blood monocytes were differentiated over six days with either 100 ng/ml M-CSF or 1 umol/l CXCL4
In atherosclerotic arteries, blood monocytes differentiate to macrophages in the presence of growth factors like macrophage colony-stimulation factor (MCSF) and chemokines like platelet factor 4 (CXCL4). To compare the gene expression signature of CXCL4-induced macrophages with MCSF-induced macrophages or macrophages polarized with IFN-γ/LPS (M1) or IL-4 (M2), we cultured primary human peripheral blood monocytes for six days. mRNA expression was measured by Affymetrix gene chips and differences were analyzed by Local Pooled Error test, Profile of Complex Functionality and Gene Set Enrichment Analysis. 375 genes were differentially expressed between MCSF- and CXCL4-induced macrophages, 206 of them overexpressed in CXCL4 macrophages coding for genes implicated in the inflammatory/immune response, antigen processing/presentation, and lipid metabolism. CXCL4-induced macrophages overexpressed some M1 and M2 genes and the corresponding cytokines at the protein level, however, their transcriptome clustered with neither M1 nor M2 transcriptomes. They almost completely lost the ability to phagocytose zymosan beads. Genes linked to atherosclerosis were not consistently up- or downregulated. Scavenger receptors showed lower and cholesterol efflux transporters higher expression in CXCL4- than MCSF-induced macrophages, resulting in lower LDL content. We conclude that CXCL4 induces a unique macrophage transcriptome distinct from known macrophage types, defining a new macrophage differentiation that we propose to call M4.
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| Overall design |
two MCSF samples and two CXCL4 samples
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| Contributor(s) |
Gleissner CA, Ley K |
| Citation(s) |
20335529 |
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| Submission date |
Feb 23, 2010 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Christian Albert Gleissner |
| E-mail(s) |
gleissner.christian@rottalinnkliniken.de
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| Phone |
+49-8721-9837302
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| Organization name |
Rottal-Inn Kliniken KU
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| Department |
Cardiology
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| Lab |
Gleissner
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| Street address |
Simonsöder Allee 20
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| City |
Eggenfelden |
| ZIP/Postal code |
84307 |
| Country |
Germany |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA125173 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20484_RAW.tar |
19.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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