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Status |
Public on Nov 08, 2022 |
Title |
Exploring differentially expressed genes in hypothalamic (HT), pars tuberalis (PT) and pineal gland (PG) transcriptomes in different sexual behaviour phenotypes in rams using RNA-seq |
Organism |
Ovis aries |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
To understand the mechanisms of sexual behaviour in rams, transcriptomic sequencing technology was used to identify differentially expressed genes in key brain tissues We then performed gene expression profiling analysis using data obtained from RNA-seq of hypothalamus (HT), pars tuberalis (PT) and pineal gland (PG) in rams with different sexual behaviours
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Overall design |
The PT, HT and PG transcriptomes of 12 Rasa Aragonesa rams (6 sexually active (A) and 6 not active (NA) for each tissue) were generated by Novaseq 6000 platform Identifcation of differentially expressed genes (DEGs) in the 3 studied tissues. The multifactorial design was used where the state (A or NA) was defined as the primary contrast condition and the tissue (PT, HT, and PG) as the secondary contrast condition
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Contributor(s) |
Lacosta JH, Lakhssassi K |
Citation(s) |
36331073 |
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Submission date |
May 26, 2022 |
Last update date |
Nov 08, 2022 |
Contact name |
Kenza Lakhssassi |
E-mail(s) |
kenza.lakhssassi@inra.ma
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Organization name |
CITA
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Street address |
Av MontaƱana, 930,
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City |
Zaragoza |
State/province |
Zaragoza |
ZIP/Postal code |
50059 |
Country |
Spain |
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Platforms (1) |
GPL27721 |
Illumina NovaSeq 6000 (Ovis aries) |
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Samples (36)
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GSM6199000 |
Hypothalamus, active, animal 1 [1_HT_A] |
GSM6199001 |
Hypothalamus, active, animal 2 [2_HT_A] |
GSM6199002 |
Hypothalamus, active, animal 3 [3_HT_A] |
GSM6199003 |
Hypothalamus, active, animal 4 [4_HT_A] |
GSM6199004 |
Hypothalamus, active, animal 5 [5_HT_A] |
GSM6199005 |
Hypothalamus, active, animal 6 [6_HT_A] |
GSM6199006 |
Hypothalamus, not active, animal 7 [7_HT_NA] |
GSM6199007 |
Hypothalamus, not active, animal 8 [8_HT_NA] |
GSM6199008 |
Hypothalamus, not active, animal 9 [9_HT_NA] |
GSM6199009 |
Hypothalamus, not active, animal 10 [10_HT_NA] |
GSM6199010 |
Hypothalamus, not active, animal 11 [11_HT_NA] |
GSM6199011 |
Hypothalamus, not active, animal 12 [12_HT_NA] |
GSM6199012 |
Pars Tuberalis, active, animal 1 [13_PT_A] |
GSM6199013 |
Pars Tuberalis, active, animal 2 [14_PT_A] |
GSM6199014 |
Pars Tuberalis, active, animal 3 [15_PT_A] |
GSM6199015 |
Pars Tuberalis, active, animal 4 [16_PT_A] |
GSM6199016 |
Pars Tuberalis, active, animal 5 [17_PT_A] |
GSM6199017 |
Pars Tuberalis, active, animal 6 [18_PT_A] |
GSM6199018 |
Pars Tuberalis, not active, animal 7 [19_PT_NA] |
GSM6199019 |
Pars Tuberalis, not active, animal 8 [20_PT_NA] |
GSM6199020 |
Pars Tuberalis, not active, animal 9 [21_PT_NA] |
GSM6199021 |
Pars Tuberalis, not active, animal 10 [22_PT_NA] |
GSM6199022 |
Pars Tuberalis, not active, animal 11 [23_PT_NA] |
GSM6199023 |
Pars Tuberalis, not active, animal 12 [24_PT_NA] |
GSM6199024 |
Pineal Gland, active, animal 1 [25_PG_A] |
GSM6199025 |
Pineal Gland, active, animal 2 [26_PG_A] |
GSM6199026 |
Pineal Gland, active, animal 3 [27_PG_A] |
GSM6199027 |
Pineal Gland, active, animal 4 [28_PG_A] |
GSM6199028 |
Pineal Gland, active, animal 5 [29_PG_A] |
GSM6199029 |
Pineal Gland, active, animal 6 [30_PG_A] |
GSM6199030 |
Pineal Gland, not active, animal 7 [31_PG_NA] |
GSM6199031 |
Pineal Gland, not active, animal 8 [32_PG_NA] |
GSM6199032 |
Pineal Gland, not active, animal 9 [33_PG_NA] |
GSM6199033 |
Pineal Gland, not active, animal 10 [34_PG_NA] |
GSM6199034 |
Pineal Gland, not active, animal 11 [35_PG_NA] |
GSM6199035 |
Pineal Gland, not active, animal 12 [36_PG_NA] |
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Relations |
BioProject |
PRJNA842577 |
Supplementary file |
Size |
Download |
File type/resource |
GSE204861_Raw_gene_counts_matrix.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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