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| Status |
Public on Sep 02, 2022 |
| Title |
Role of ZNF281 in intestinal fibroblast activation: new insights in IBD-related fibrosis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Serious complications of Crohn’s disease (CD), a form of Inflammatory Bowel Disease (IBD), involve the formation of intestinal fibrotic stenosis followed by obstruction. Chronic inflammatory state activates resident fibroblasts and myofibroblasts, that are the key effector cells in fibrosis, being responsible for the synthesis of ECM proteins.
Exposure of human colon fibroblasts (CCD18-Co) to the pro-fibrotic cytokine TGF-β1 is sufficient to induce the expression of genes associated with myofibroblast features such as cell migration, ECM remodeling and contraction. Furthermore, during TGFβ1-mediated myofibroblast differentiation, the transcription factor ZNF281 is increased and is required to reshape the expression of genes encoding for myofibroblast markers, suggesting an active role for ZNF281 as a master regulator of intestinal myofibroblast functions.
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| Overall design |
12 samples
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| Contributor(s) |
Fulci V, Laudadio I, Stronati L |
| Citation(s) |
36142169 |
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| Submission date |
May 26, 2022 |
| Last update date |
Oct 04, 2022 |
| Contact name |
valerio fulci |
| Organization name |
Università i Roma "La Sapienza"
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| Department |
Dipartimento di Medicina Molecolare
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| Street address |
Vle regina Elena, 324
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| City |
Roma |
| ZIP/Postal code |
00161 |
| Country |
Italy |
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| Platforms (1) |
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| Samples (12)
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| GSM6199145 |
CCD-18Co cells, CTRL siRNA, biol rep 1 |
| GSM6199146 |
CCD-18Co cells, TGF-beta, CTRL siRNA, biol rep 1 |
| GSM6199147 |
CCD-18Co cells, ZNF281 siRNA, biol rep 1 |
| GSM6199148 |
CCD-18Co cells, TGF-beta, ZNF281 siRNA, biol rep 1 |
| GSM6199149 |
CCD-18Co cells, CTRL siRNA, biol rep 2 |
| GSM6199150 |
CCD-18Co cells, TGF-beta, CTRL siRNA, biol rep 2 |
| GSM6199151 |
CCD-18Co cells, ZNF281 siRNA, biol rep 2 |
| GSM6199152 |
CCD-18Co cells, TGF-beta, ZNF281 siRNA, biol rep 2 |
| GSM6199153 |
CCD-18Co cells, CTRL siRNA, biol rep 3 |
| GSM6199154 |
CCD-18Co cells, TGF-beta, CTRL siRNA, biol rep 3 |
| GSM6199155 |
CCD-18Co cells, ZNF281 siRNA, biol rep 3 |
| GSM6199156 |
CCD-18Co cells, TGF-beta, ZNF281 siRNA, biol rep 3 |
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| Relations |
| BioProject |
PRJNA842655 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE204868_RAW.tar |
2.7 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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