|
| Status |
Public on May 30, 2022 |
| Title |
Gene expression profiling of NUAK kinase overexpression in Drosophila larval muscle development |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Signal transduction pathways mediated by kinases control diverse biological outputs at the level of cells and tissues to regulate a diverse array of biological and developmental events. To gain insight into how muscle expression of the evolutionarily conserved NUAK kinase regulates the transcriptional landscape during Drosophila melanogaster development, we performed high-throughput sequencing of RNA from either whole larvae or dissected muscle fillets at the end of larval development. Raw data was generated using the Illumina HiSeq platform. After trimming and mapping to the Drosophila reference genome, differential gene expression and GO enrichment analysis were completed.
|
| |
|
| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for mef2>lacZ control or NUAK (mef2>NUAK 548 or mef2>NUAK 550) overexpression samples.
grant information: RO1 AR060788
Mechanisms Underlying Muscle Development and Maintenance in Drosophila, NIH/NIAMS
|
| |
|
| Contributor(s) |
Brooks D, Geisbrecht ER |
| Citation(s) |
35924094, 35760368 |
| |
| Submission date |
May 26, 2022 |
| Last update date |
Nov 29, 2022 |
| Contact name |
Erika R Geisbrecht |
| E-mail(s) |
geisbrechte@ksu.edu
|
| Phone |
785-532-3105
|
| Organization name |
Kansas State University
|
| Department |
Biochemistry and Molecular Biophysics
|
| Street address |
141 Chalmers Hall, Chalmers Hall
|
| City |
Manhattan |
| State/province |
KS |
| ZIP/Postal code |
66506-8008 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21306 |
Illumina HiSeq 4000 (Drosophila melanogaster) |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA842684 |
Less...
More...